@article {4446,
	title = {Characterization and adaptive optical correction of aberrations during in vivo imaging in the mouse cortex.},
	journal = {Proceedings of the National Academy of Sciences of the United States of America},
	volume = {109},
	year = {2012},
	month = {2012 Jan 3},
	pages = {22-7},
	abstract = {<p>The signal and resolution during in vivo imaging of the mouse brain is limited by sample-induced optical aberrations. We find that, although the optical aberrations can vary across the sample and increase in magnitude with depth, they remain stable for hours. As a result, two-photon adaptive optics can recover diffraction-limited performance to depths of 450\&nbsp;μm and improve imaging quality over fields of view of hundreds of microns. Adaptive optical correction yielded fivefold signal enhancement for small neuronal structures and a threefold increase in axial resolution. The corrections allowed us to detect smaller neuronal structures at greater contrast and also improve the signal-to-noise ratio during functional Ca(2+) imaging in single neurons.</p>
},
	issn = {1091-6490},
	doi = {10.1073/pnas.1109202108},
	author = {Ji, Na and Sato, Takashi R and Betzig, Eric}
}
