@article {6293,
	title = {Noninvasive imaging beyond the diffraction limit of 3D dynamics in thickly fluorescent specimens.},
	journal = {Cell},
	volume = {151},
	year = {2012},
	month = {2012 Dec 7},
	pages = {1370-85},
	abstract = {<p>Optical imaging of the dynamics of living specimens involves tradeoffs between spatial resolution, temporal resolution, and phototoxicity, made more difficult in three dimensions. Here, however, we report that rapid three-dimensional (3D) dynamics can be studied beyond the diffraction limit in thick or densely fluorescent living specimens over many time points by combining ultrathin planar illumination\&nbsp;produced by scanned Bessel beams with super-resolution structured illumination microscopy. We demonstrate in\&nbsp;vivo karyotyping of chromosomes during mitosis and identify different dynamics for the actin cytoskeleton at the dorsal and ventral surfaces of fibroblasts. Compared to spinning disk confocal microscopy, we\&nbsp;demonstrate substantially reduced photodamage when imaging rapid morphological changes in D.\&nbsp;discoideum cells, as well as improved contrast and resolution at depth within developing C.\&nbsp;elegans embryos. Bessel beam structured plane illumination thus promises new insights into complex biological phenomena that require 4D subcellular spatiotemporal detail in either a single or multicellular context.</p>
},
	keywords = {Animals, Brain, Caenorhabditis elegans, Cell Line, Dermatitis, Dictyostelium, Drosophila melanogaster, Fibroblasts, Fluorescence, Humans, Imaging, Karyotyping, Larva, Microscopy, Mitosis, Optical Imaging, Phototoxic, Three-Dimensional, Tumor},
	issn = {1097-4172},
	doi = {10.1016/j.cell.2012.10.008},
	author = {Gao, Liang and Shao, Lin and Higgins, Christopher D and Poulton, John S and Peifer, Mark and Davidson, Michael W and Wu, Xufeng and Goldstein, Bob and Betzig, Eric}
}
