@article {1541,
	title = {Nodavirus-induced membrane rearrangement in replication complex assembly requires replicase protein a, RNA templates, and polymerase activity.},
	journal = {Journal of Virology},
	volume = {84},
	year = {2010},
	month = {2010 Dec},
	pages = {12492-503},
	abstract = {<p>Positive-strand RNA [(+)RNA] viruses invariably replicate their RNA genomes on modified intracellular membranes. In infected Drosophila cells, Flock House nodavirus (FHV) RNA replication complexes form on outer mitochondrial membranes inside \~{}50-nm, virus-induced spherular invaginations similar to RNA replication-linked spherules induced by many (+)RNA viruses at various membranes. To better understand replication complex assembly, we studied the mechanisms of FHV spherule formation. FHV has two genomic RNAs; RNA1 encodes multifunctional RNA replication protein A and RNA interference suppressor protein B2, while RNA2 encodes the capsid proteins. Expressing genomic RNA1 without RNA2 induced mitochondrial spherules indistinguishable from those in FHV infection. RNA1 mutation showed that protein B2 was dispensable and that protein A was the only FHV protein required for spherule formation. However, expressing protein A alone only "zippered" together the surfaces of adjacent mitochondria, without inducing spherules. Thus, protein A is necessary but not sufficient for spherule formation. Coexpressing protein A plus a replication-competent FHV RNA template induced RNA replication in trans and membrane spherules. Moreover, spherules were not formed when replicatable FHV RNA templates were expressed with protein A bearing a single, polymerase-inactivating amino acid change or when wild-type protein A was expressed with a nonreplicatable FHV RNA template. Thus, unlike many (+)RNA viruses, the membrane-bounded compartments in which FHV RNA replication occurs are not induced solely by viral protein(s) but require viral RNA synthesis. In addition to replication complex assembly, the results have implications for nodavirus interaction with cell RNA silencing pathways and other aspects of virus control.</p>
},
	keywords = {Animals, Blotting, Capsid Proteins, DNA-Directed RNA Polymerases, Drosophila melanogaster, Genome, Intracellular Membranes, Mitochondria, Nodaviridae, Northern, Plasmids, RNA, RNA Replicase, RNA Virus Infections, Small Interfering, Viral, Virus Replication, Western},
	issn = {1098-5514},
	doi = {10.1128/JVI.01495-10},
	url = {http://jvi.asm.org/content/84/24/12492.short},
	author = {Kopek, Benjamin G and Settles, Erik W and Friesen, Paul D and Ahlquist, Paul}
}
