@article {2443,
	title = {Molecular patterning mechanism underlying metamorphosis of the thoracic leg in Manduca sexta.},
	journal = {Developmental Biology},
	volume = {305},
	year = {2007},
	month = {2007 May 15},
	pages = {539-50},
	abstract = {<p>The tobacco hornworm Manduca sexta, like many holometabolous insects, makes two versions of its thoracic legs. The simple legs of the larva are formed during embryogenesis, but then are transformed into the more complex adult legs at metamorphosis. To elucidate the molecular patterning mechanism underlying this biphasic development, we examined the expression patterns of five genes known to be involved in patterning the proximal-distal axis in insect legs. In the developing larval leg of Manduca, the early patterning genes Distal-less and Extradenticle are already expressed in patterns comparable to the adult legs of other insects. In contrast, Bric-a-brac and dachshund are expressed in patterns similar to transient patterns observed during early stages of leg development in Drosophila. During metamorphosis of the leg, the two genes finally develop mature expression patterns. Our results are consistent with the hypothesis that the larval leg morphology is produced by a transient arrest in the conserved adult leg patterning process in insects. In addition, we find that, during the adult leg development, some cells in the leg express the patterning genes de novo suggesting that the remodeling of the leg involves changes in the patterning gene regulation.</p>
},
	keywords = {Animals, Biological, Body Patterning, Extremities, Larva, Manduca, Metamorphosis, Thorax},
	issn = {0012-1606},
	doi = {10.1016/j.ydbio.2007.02.042},
	author = {Tanaka, Kohtaro and Truman, James W}
}
