@article {1586,
	title = {Identification of a glycolytic regulon in the archaea Pyrococcus and Thermococcus.},
	journal = {FEMS Microbiology Letters},
	volume = {260},
	year = {2006},
	month = {2006 Jul},
	pages = {69-76},
	abstract = {<p>The glycolytic pathway of the hyperthermophilic archaea that belong to the order Thermococcales (Pyrococcus, Thermococcus and Palaeococcus) differs significantly from the canonical Embden-Meyerhof pathway in bacteria and eukarya. This archaeal glycolysis variant consists of several novel enzymes, some of which catalyze unique conversions. Moreover, the enzymes appear not to be regulated allosterically, but rather at transcriptional level. To elucidate details of the gene expression control, the transcription initiation sites of the glycolytic genes in Pyrococcus furiosus have been mapped by primer extension analysis and the obtained promoter sequences have been compared with upstream regions of non-glycolytic genes. Apart from consensus sequences for the general transcription factors (TATA-box and BRE) this analysis revealed the presence of a potential transcription factor binding site (TATCAC-N(5)-GTGATA) in glycolytic and starch utilizing promoters of P. furiosus and several thermococcal species. The absence of this inverted repeat in Pyrococcus abyssi and Pyrococcus horikoshii probably reflects that their reduced catabolic capacity does not require this regulatory system. Moreover, this phyletic pattern revealed a TrmB-like regulator (PF0124 and TK1769) which may be involved in recognizing the repeat. This Thermococcales glycolytic regulon, with more than 20 genes, is the largest regulon that has yet been described for Archaea.</p>
},
	keywords = {Base Sequence, Binding Sites, Genetic, Glycolysis, Molecular Sequence Data, Promoter Regions, Pyrococcus, Regulon, Thermococcus, Transcription Initiation Site},
	issn = {0378-1097},
	doi = {10.1111/j.1574-6968.2006.00292.x},
	author = {van de Werken, Harmen J G and Verhees, Corn{\'e} H and Akerboom, Jasper and de Vos, Willem M and van der Oost, John}
}
