@article {1170,
	title = {A persistent RNA-DNA hybrid is formed during transcription at a phylogenetically conserved mitochondrial DNA sequence.},
	journal = {Molecular and Cellular Biology},
	volume = {15},
	year = {1995},
	month = {1995 Jan},
	pages = {580-9},
	abstract = {<p>Critical features of the mitochondrial leading-strand DNA replication origin are conserved from Saccharomyces cerevisiae to humans. These include a promoter and a downstream GC-rich sequence block (CSBII) that encodes rGs within the primer RNA. During in vitro transcription at yeast mitochondrial replication origins, there is stable and persistent RNA-DNA hybrid formation that begins at the 5{\textquoteright} end of the rG region. The short rG-dC sequence is the necessary and sufficient nucleic acid element for establishing stable hybrids, and the presence of rGs within the RNA strand of the RNA-DNA hybrid is required. The efficiency of hybrid formation depends on the length of RNA synthesized 5{\textquoteright} to CSBII and the type of RNA polymerase employed. Once made, the RNA strand of an RNA-DNA hybrid can serve as an effective primer for mitochondrial DNA polymerase. These results reveal a new mechanism for persistent RNA-DNA hybrid formation and suggest a step in priming mitochondrial DNA replication that requires both mitochondrial RNA polymerase and an rG-dC sequence-specific event to form an extensive RNA-DNA hybrid.</p>
},
	keywords = {Base Sequence, DNA, DNA Primers, DNA Replication, DNA-Directed DNA Polymerase, DNA-Directed RNA Polymerases, Genetic, Mitochondrial, Molecular Sequence Data, Nucleic Acid, Nucleic Acid Hybridization, Phylogeny, Regulatory Sequences, Ribonuclease H, RNA, Saccharomyces cerevisiae, Transcription},
	issn = {0270-7306},
	doi = {10.1101/gad.1352105},
	author = {Xu, B and Clayton, D A}
}
