@article {45783,
	title = {Isolation of mitochondria from tissue culture cells.},
	journal = {Cold Spring Harbor Protocols},
	volume = {2014},
	year = {2014},
	month = {2014 Oct},
	pages = {pdb.prot080002},
	abstract = {<p>The number of mitochondria per cell varies substantially from cell line to cell line. For example, human HeLa cells contain at least twice as many mitochondria as smaller mouse L cells. This protocol starts with a washed cell pellet of 1-2 mL derived from \~{}10$^{9}$ cells grown in culture. The cells are swollen in a hypotonic buffer and ruptured with a Dounce or Potter-Elvehjem homogenizer using a tight-fitting pestle, and mitochondria are isolated by differential centrifugation.</p>
},
	issn = {1559-6095},
	doi = {10.1101/pdb.prot080002},
	author = {Clayton, David A and Shadel, Gerald S}
}
