@article {45844,
	title = {Fixation-resistant photoactivatable fluorescent proteins for CLEM.},
	journal = {Nature Methods},
	volume = {12},
	year = {2015},
	month = {2015 Mar},
	pages = {215-8},
	abstract = {<p>Fluorescent proteins facilitate a variety of imaging paradigms in live and fixed samples. However, they lose their fluorescence after heavy fixation, hindering applications such as correlative light and electron microscopy (CLEM). Here we report engineered variants of the photoconvertible Eos fluorescent protein that fluoresce and photoconvert normally in heavily fixed (0.5-1\% OsO4), plastic resin-embedded samples, enabling correlative super-resolution fluorescence imaging and high-quality electron microscopy.</p>
},
	issn = {1548-7105},
	doi = {10.1038/nmeth.3225},
	author = {Paez-Segala, Maria G and Sun, Mei G and Shtengel, Gleb and Viswanathan, Sarada and Baird, Michelle A and Macklin, John J and Patel, Ronak and Allen, John R and Howe, Elizabeth S and Piszczek, Grzegorz and Hess, Harald F and Davidson, Michael W and Wang, Yalin and Looger, Loren L}
}
