@article {46721,
	title = {β-Actin mRNA compartmentalization enhances focal adhesion stability and directs cell migration.},
	journal = {Genes \& Development},
	volume = {26},
	year = {2012},
	month = {2012 Sep 1},
	pages = {1885-90},
	abstract = {<p>Directed cell motility is at the basis of biological phenomena such as development, wound healing, and metastasis. It has been shown that substrate attachments mediate motility by coupling the cell{\textquoteright}s cytoskeleton with force generation. However, it has been unclear how the persistence of cell directionality is facilitated. We show that mRNA localization plays an important role in this process, but the mechanism of action is still unknown. In this study, we show that the zipcode-binding protein 1 transports β-actin mRNA to the focal adhesion compartment, where it dwells for minutes, suggesting a means for associating its localization with motility through the formation of stable connections between adhesions and newly synthesized actin filaments. In order to demonstrate this, we developed an approach for assessing the functional consequences of β-actin mRNA and protein localization by tethering the mRNA to a specific location-in this case, the focal adhesion complex. This approach will have a significant impact on cell biology because it is now possible to forcibly direct any mRNA and its cognate protein to specific locations in the cell. This will reveal the importance of localized protein translation on various cellular processes.</p>
},
	keywords = {Actins, Animals, Cell Compartmentation, Cell Line, Cell Movement, Cells, Cultured, Fibroblasts, Focal Adhesions, Glycoproteins, Mice, Mice, Knockout, RNA, Messenger},
	issn = {1549-5477},
	doi = {10.1101/gad.190413.112},
	author = {Katz, Zachary B and Wells, Amber L and Park, Hye Yoon and Wu, Bin and Shenoy, Shailesh M and Singer, Robert H}
}
