@article {47663,
	title = {Functional nanoscale organization of signaling molecules downstream of the T cell antigen receptor.},
	journal = {Immunity},
	volume = {35},
	year = {2011},
	month = {2011 Nov 23},
	pages = {705-20},
	abstract = {<p>Receptor-regulated cellular signaling often is mediated by formation of transient, heterogeneous protein complexes of undefined structure. We used single and two-color photoactivated localization microscopy to study complexes downstream of the T cell antigen receptor (TCR) in single-molecule detail at the plasma membrane of intact T cells. The kinase ZAP-70 distributed completely with the TCRζ chain and both partially mixed with the adaptor LAT in activated cells, thus showing localized activation of LAT by TCR-coupled ZAP-70. In resting and activated cells, LAT primarily resided in nanoscale clusters as small as dimers whose formation depended on protein-protein and protein-lipid interactions. Surprisingly, the adaptor SLP-76 localized to the periphery of LAT clusters. This nanoscale structure depended on polymerized actin and its disruption affected TCR-dependent cell function. These results extend our understanding of the mechanism of T cell activation and the formation and organization of TCR-mediated signaling complexes, findings also relevant to other receptor systems.</p>
},
	keywords = {Actins, Adaptor Proteins, Signal Transducing, Cell Line, Tumor, Cells, Cultured, Humans, Jurkat Cells, Lymphocyte Activation, Membrane Proteins, Phospholipase C gamma, Phosphoproteins, Phosphorylation, Protein Binding, Protein Multimerization, Protein Transport, Receptors, Antigen, T-Cell, Receptors, Antigen, T-Cell, gamma-delta, Signal Transduction, T-Lymphocytes, ZAP-70 Protein-Tyrosine Kinase},
	issn = {1097-4180},
	doi = {10.1016/j.immuni.2011.10.004},
	author = {Sherman, Eilon and Barr, Valarie and Manley, Suliana and Patterson, George and Balagopalan, Lakshmi and Akpan, Itoro and Regan, Carole K and Merrill, Robert K and Sommers, Connie L and Lippincott-Schwartz, Jennifer and Samelson, Lawrence E}
}
