@article {47683,
	title = {Superresolution imaging using single-molecule localization.},
	journal = {Annual review of physical chemistry},
	volume = {61},
	year = {2010},
	month = {2010},
	pages = {345-67},
	abstract = {<p>Superresolution imaging is a rapidly emerging new field of microscopy that dramatically improves the spatial resolution of light microscopy by over an order of magnitude (approximately 10-20-nm resolution), allowing biological processes to be described at the molecular scale. Here, we discuss a form of superresolution microscopy based on the controlled activation and sampling of sparse subsets of photoconvertible fluorescent molecules. In this single-molecule-based imaging approach, a wide variety of probes have proved valuable, ranging from genetically encodable photoactivatable fluorescent proteins to photoswitchable cyanine dyes. These have been used in diverse applications of superresolution imaging: from three-dimensional, multicolor molecule localization to tracking of nanometric structures and molecules in living cells. Single-molecule-based superresolution imaging thus offers exciting possibilities for obtaining molecular-scale information on biological events occurring at variable timescales.</p>
},
	keywords = {Image Interpretation, Computer-Assisted, Luminescent Proteins, Microscopy, Fluorescence, Microscopy, Polarization},
	issn = {1545-1593},
	doi = {10.1146/annurev.physchem.012809.103444},
	author = {Patterson, George and Davidson, Michael and Manley, Suliana and Lippincott-Schwartz, Jennifer}
}
