@article {48559,
	title = {Quantitative mRNA imaging throughout the entire Drosophila brain.},
	journal = {Nature Methods},
	volume = {14},
	year = {2017},
	month = {2017 Jun 05},
	pages = {703-6},
	abstract = {<p>We describe a fluorescence in situ hybridization method that permits detection of the localization and abundance of single mRNAs (smFISH) in cleared whole-mount adult Drosophila brains. The approach is rapid and multiplexable and does not require molecular amplification; it allows facile quantification of mRNA expression with subcellular resolution on a standard confocal microscope. We further demonstrate single-mRNA detection across the entire brain using a custom Bessel beam structured illumination microscope (BB-SIM).</p>
},
	issn = {1548-7105},
	doi = {10.1038/nmeth.4309},
	author = {Long, Xi and Colonell, Jennifer and Wong, Allan M and Singer, Robert H and Lionnet, Timoth{\'e}e}
}
