@article {48604,
	title = {A method to minimize condenser lens-induced hysteresis effects in a JEOL JEM-3200FSC microscope to enable stable cryoEM low-dose operations.},
	journal = {bioRxiv},
	year = {2017},
	month = {2017 Jun 21},
	abstract = {<p>Low dose imaging procedures are key for a successful cryoEM experiment (whether by electron cryotomography, single particle analysis, electron crystallography, or MicroED). We present a method to minimize magnetic hysteresis of the condenser lens system in the JEOL JEM-3200FSC transmission electron microscope (TEM) in order to maintain a stable optical axis for the beam path of low-dose imaging. The simple procedure involves independent voltage ramping of the CL1 and CL2 lenses immediately before switching to the focusing and exposure beam settings for data collection.</p>
},
	doi = {10.1101/153395},
	author = {de la Cruz, Michael Jason and Martynowycz, Michael and Hattne, Johan and Shi, Dan and Gonen, Tamir}
}
