@inbook {48701,
	title = {Recent progress in the 3D reconstruction of Drosophila neural circuits.},
	booktitle = {Decoding Neural Circuit Structure and Function},
	year = {2017},
	pages = {63-89},
	publisher = {Springer},
	organization = {Springer},
	abstract = {<p>The brain of fruit fly Drosophila melanogaster has been used as a model system for functional analysis of neuronal circuits, including connectomics research, due to its modest size (~700\&nbsp;μm) and availability of abundant molecular genetics tools for visualizing neurons. Three-dimensional (3D) reconstruction of high-resolution images of neurons or circuits visualized with appropriate methods is a critical step for obtaining information such as morphology and connectivity patterns of neuronal circuits. In this chapter, we introduce methods for generating 3D reconstructed images with data acquired from confocal laser scanning microscopy (CLSM) or electron microscopy (EM) to analyze neuronal circuits found in the central nervous system (CNS) of the fruit fly. Comparisons of different algorithms and strategies for reconstructing neuronal circuits, using actual studies as references, will be discussed within this chapter.</p>
},
	doi = {10.1007/978-3-319-57363-2_3},
	author = {Shinomiya, Kazunori and Ito, Masayoshi}
}
