@article {48804,
	title = {Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and Their Use in Cellular Imaging Experiments.},
	journal = {Methods in Molecular Biology (Clifton, N.J.)},
	volume = {1663},
	year = {2017},
	month = {2017},
	pages = {179-188},
	abstract = {<p>The development of genetically encoded self-labeling protein tags such as the HaloTag and SNAP-tag has expanded the utility of chemical dyes in microscopy. Intracellular labeling using these systems requires small, cell-permeable dyes with high brightness and photostability. We recently discovered a general method to improve the properties of classic fluorophores by replacing N,N-dimethylamino groups with four-membered azetidine rings to create the "Janelia Fluor" dyes. Here, we describe the synthesis of the HaloTag and SNAP-tag ligands of Janelia Fluor 549 and Janelia Fluor 646 as well as standard labeling protocols for use in ensemble and single-molecule cellular imaging.</p>
},
	issn = {1940-6029},
	doi = {10.1007/978-1-4939-7265-4_15},
	author = {Grimm, Jonathan B and Brown, Timothy A and English, Brian P and Lionnet, Timoth{\'e}e and Lavis, Luke D}
}
