@article {49343,
	title = {Inverse-response Ca2+ indicators for optogenetic visualization of neuronal inhibition.},
	journal = {Scientific Reports},
	volume = {8},
	year = {2018},
	month = {2018 Aug 06},
	pages = {11758},
	abstract = {<p>We have developed a series of yellow genetically encoded Ca indicators for optical imaging (Y-GECOs) with inverted responses to Ca and apparent dissociation constants (K{\textquoteright}) ranging from 25 to 2400 nM. To demonstrate the utility of this affinity series of Ca indicators, we expressed the four highest affinity variants (K{\textquoteright}s = 25, 63, 121, and 190 nM) in the Drosophila medulla intrinsic neuron Mi1. Hyperpolarization of Mi1 by optogenetic stimulation of the laminar monopolar neuron L1 produced a decrease in intracellular Ca in layers 8-10, and a corresponding increase in Y-GECO fluorescence. These experiments revealed that lower K{\textquoteright} was associated with greater increases in fluorescence, but longer delays to reach the maximum signal change due to slower off-rate kinetics.</p>
},
	issn = {2045-2322},
	doi = {10.1038/s41598-018-30080-x},
	author = {Zhao, Yufeng and Bushey, Daniel and Zhao, Yongxin and Schreiter, Eric R and Harrison, D Jed and Wong, Allan M and Campbell, Robert E}
}
