@article {65043,
	title = {Direct wavefront sensing enables functional imaging of infragranular axons and spines.},
	journal = {Nature Methods},
	volume = {16},
	year = {2019},
	month = {2019 Jul},
	pages = {615-618},
	abstract = {<p>We advance two-photon microscopy for near-diffraction-limited imaging up to 850 {\textmu}m below the pia in awake mice. Our approach combines direct wavefront sensing of light from a guidestar (formed by descanned fluorescence from Cy5.5-conjugated dextran in brain microvessels) with adaptive optics to compensate for tissue-induced aberrations in the wavefront. We achieve high signal-to-noise ratios in recordings of glutamate release from thalamocortical axons and calcium transients in spines of layer 5b basal dendrites during active tactile sensing.</p>
},
	issn = {1548-7105},
	doi = {10.1038/s41592-019-0434-7},
	author = {Liu, Rui and Li, Zengyi and Marvin, Jonathan S and Kleinfeld, David}
}
