@article {65150,
	title = {Isomeric tuning yields bright and targetable red Ca indicators.},
	journal = {Journal of the American Chemical Society},
	volume = {141},
	year = {2019},
	month = {2019 Sep 04},
	pages = {13734-13738},
	abstract = {<p>Targeting small-molecule fluorescent indicators using genetically encoded protein tags yields new hybrid sensors for biological imaging. Optimization of such systems requires redesign of the synthetic indicator to allow cell-specific targeting without compromising the photophysical properties or cellular performance of the small-molecule probe. We developed a bright and sensitive Ca indicator by systematically exploring the relative configuration of dye and chelator, which can be targeted using the HaloTag self-labeling tag system. Our "isomeric tuning" approach is generalizable, yielding a far-red targetable indicator to visualize Ca fluxes in the primary cilium.</p>
},
	issn = {1520-5126},
	doi = {10.1021/jacs.9b06092},
	author = {Deo, Claire and Sheu, Shu-Hsien and Seo, Jinyoung and Clapham, David E and Lavis, Luke D}
}
