@article {65235,
	title = {In situ structure determination at nanometer resolution using TYGRESS.},
	journal = {Nature Methods},
	volume = {17},
	year = {2020},
	month = {2020 Feb 01},
	pages = {201-08},
	abstract = {<p>The resolution of subtomogram averages calculated from cryo-electron tomograms (cryo-ET) of crowded cellular environments is often limited owing to signal loss in, and misalignment of, the subtomograms. By contrast, single-particle cryo-electron microscopy (SP-cryo-EM) routinely reaches near-atomic resolution of isolated complexes. We report a method called {\textquoteright}tomography-guided 3D reconstruction of subcellular structures{\textquoteright} (TYGRESS) that is a hybrid of cryo-ET and SP-cryo-EM, and is able to achieve close-to-nanometer resolution of complexes inside crowded cellular environments. TYGRESS combines the advantages of SP-cryo-EM (images with good signal-to-noise ratio and contrast, as well as minimal radiation damage) and subtomogram averaging (three-dimensional alignment of macromolecules in a complex sample). Using TYGRESS, we determined the structure of the intact ciliary axoneme with up to resolution of 12 {\r A}. These results reveal many structural details that were not visible by cryo-ET alone. TYGRESS is generally applicable to cellular complexes that are amenable to subtomogram averaging.</p>
},
	issn = {1548-7105},
	doi = {10.1038/s41592-019-0651-0},
	author = {Song, Kangkang and Shang, Zhiguo and Fu, Xiaofeng and Lou, Xiaochu and Grigorieff, Nikolaus and Nicastro, Daniela}
}
