@article {65371,
	title = {CAMIO: a transgenic CRISPR pipeline to create diverse targeted genome deletions in Drosophila.},
	journal = {Nucleic Acids Research},
	year = {2020},
	month = {2020 Mar 18},
	abstract = {<p>The genome is the blueprint for an organism. Interrogating the genome, especially locating critical cis-regulatory elements, requires deletion analysis. This is conventionally performed using synthetic constructs, making it cumbersome and non-physiological. Thus, we created Cas9-mediated Arrayed Mutagenesis of Individual Offspring (CAMIO) to achieve comprehensive analysis of a targeted region of native DNA. CAMIO utilizes CRISPR that is spatially restricted to generate independent deletions in the intact Drosophila genome. Controlled by recombination, a single guide RNA is stochastically chosen from a set targeting a specific DNA region. Combining two sets increases variability, leading to either indels at 1-2 target sites or inter-target deletions. Cas9 restriction to male germ cells elicits autonomous double-strand-break repair, consequently creating offspring with diverse mutations. Thus, from a single population cross, we can obtain a deletion matrix covering a large expanse of DNA at both coarse and fine resolution. We demonstrate the ease and power of CAMIO by mapping 5{\textquoteright}UTR sequences crucial for chinmo{\textquoteright}s post-transcriptional regulation.</p>
},
	issn = {1362-4962},
	doi = {10.1093/nar/gkaa177},
	author = {Chen, Hui-Min and Marques, Jorge Garcia and Sugino, Ken and Wei, Dingjun and Miyares, Rosa Linda and Lee, Tzumin}
}
