@article {65763,
	title = {A far-red fluorescent chemogenetic reporter for in vivo molecular imaging},
	journal = {Angewandte Chemie International Edition},
	year = {2020},
	month = {06/2020},
	abstract = {<p>Far-red emitting fluorescent labels are highly desirable for spectral multiplexing and deep tissue imaging. Here, we describe the generation of frFAST (far-red Fluorescence Activating and absorption Shifting Tag), a 14-kDa monomeric protein that forms a bright far-red fluorescent assembly with (4-hydroxy-3-methoxy-phenyl)allylidene rhodanine (HPAR-3OM). As HPAR-3OM is essentially non- fluorescent in solution and in cells, frFAST can be imaged with high contrast in presence of free HPAR-3OM, which allowed the rapid and efficient imaging of frFAST fusions in live cells, zebrafish embryo/larvae and chicken embryo. Beyond enabling genetic encoding of far-red fluorescence, frFAST allowed the design of a far- red chemogenetic reporter of protein-protein interactions, demonstrating its great potential for the design of innovative far-red emitting biosensors.</p>
},
	issn = {1433-7851},
	doi = {10.1002/anie.202006576},
	author = {Li, Chenge and Tebo, Alison G and Thauvin, Marion and Plamont, Marie-Aude and Volovitch, Michel and Morin, Xavier and Vriz, Sophie and Gautier, Arnaud}
}
