@article {65798,
	title = {Behavioral state coding by molecularly defined paraventricular hypothalamic cell type ensembles.},
	journal = {Science},
	volume = {370},
	year = {2020},
	month = {2020 Oct 16},
	abstract = {<p>Brains encode behaviors using neurons amenable to systematic classification by gene expression. The contribution of molecular identity to neural coding is not understood because of the challenges involved with measuring neural dynamics and molecular information from the same cells. We developed CaRMA (calcium and RNA multiplexed activity) imaging based on recording in vivo single-neuron calcium dynamics followed by gene expression analysis. We simultaneously monitored activity in hundreds of neurons in mouse paraventricular hypothalamus (PVH). Combinations of cell-type marker genes had predictive power for neuronal responses across 11 behavioral states. The PVH uses combinatorial assemblies of molecularly defined neuron populations for grouped-ensemble coding of survival behaviors. The neuropeptide receptor neuropeptide Y receptor type 1 (Npy1r) amalgamated multiple cell types with similar responses. Our results show that molecularly defined neurons are important processing units for brain function.</p>
},
	issn = {1095-9203},
	doi = {10.1126/science.abb2494},
	author = {Xu, Shengjin and Yang, Hui and Menon, Vilas and Lemire, Andrew L and Wang, Lihua and Henry, Fredrick E and Turaga, Srinivas C and Sternson, Scott M}
}
