@article {67833,
	title = {Versatile On-Demand Fluorescent Labeling of Fusion Proteins Using Fluorescence-Activating and Absorption-Shifting Tag (FAST).},
	journal = {Methods Mol Biol},
	volume = {2350},
	year = {2021},
	month = {2021},
	pages = {253-265},
	abstract = {<p>Observing the localization, the concentration, and the distribution of proteins in cells or organisms is essential to understand theirs functions. General and versatile methods allowing multiplexed imaging of proteins under a large variety of experimental conditions are thus essential for deciphering the inner workings of cells and organisms. Here, we present a general method based on the non-covalent labeling of a small protein tag, named FAST (fluorescence-activating and absorption-shifting tag), with various fluorogenic ligands that light up upon labeling, which makes the simple, robust, and versatile on-demand labeling of fusion proteins in a wide range of experimental systems possible.</p>
},
	keywords = {Animals, Cell Line, Flow Cytometry, Fluorescent Dyes, Humans, Microscopy, Fluorescence, Molecular Structure, Recombinant Fusion Proteins, Staining and Labeling, Zebrafish},
	issn = {1940-6029},
	doi = {10.1007/978-1-0716-1593-5_16},
	author = {Gautier, Arnaud and Jullien, Ludovic and Li, Chenge and Plamont, Marie-Aude and Tebo, Alison G and Thauvin, Marion and Volovitch, Michel and Vriz, Sophie}
}
