@article {68194,
	title = {Multifunctional fluorophores for live-cell imaging and affinity capture of proteins},
	journal = {bioRxiv},
	year = {2022},
	month = {2022 Jul 03},
	abstract = {<p>The development of enzyme-based self-labeling tags allow the labeling of proteins in living cells with synthetic small-molecules. Use of a fluorophore-containing ligand enables the visualization of protein location inside cells using fluorescence microscopy. Alternatively, deployment of a biotin-containing ligand allows purification of tagged protein using affinity resins. Despite these various applications of self-labeling tags, most ligands serve a single purpose. Here, we describe self-labeling tag ligands that allow both visualization and subsequent capture of a protein. A key design principle is exploiting the chemical properties and size of a rhodamine fluorophore to optimize cell-permeability of the ligand and the capture efficiency of the biotin conjugate. This work generates useful {\textquotedblleft}multifunctional{\textquotedblright} fluorophores with generalizable design principles that will allow the construction of new tools for biology.</p>
},
	doi = {10.1101/2022.07.02.498544},
	url = {https://doi.org/10.1101/2022.07.02.498544},
	author = {Kumar, Pratik and Jason D. Vevea and Edwin R. Chapman and Luke D. Lavis}
}
