@article {68431,
	title = {Functional divergence of Plexin B structural motifs in distinct steps of  olfactory circuit assembly.},
	journal = {Elife},
	volume = {8},
	year = {2019},
	month = {06/2019},
	abstract = {<p>Plexins exhibit multitudinous, evolutionarily conserved functions in neural development. How Plexins employ their diverse structural motifs in vivo to perform distinct roles is unclear. We previously reported that Plexin B (PlexB) controls multiple steps during the assembly of the olfactory circuit (Li et al., 2018b). Here, we systematically mutagenized structural motifs of PlexB and examined the function of these variants in these multiple steps: axon fasciculation, trajectory choice, and synaptic partner selection. We found that the extracellular Sema domain is essential for all three steps, the catalytic site of the intracellular RapGAP is engaged in none, and the intracellular GTPase-binding motifs are essential for trajectory choice and synaptic partner selection, but are dispensable for fasciculation. Moreover, extracellular PlexB cleavage serves as a regulatory mechanism of PlexB signaling. Thus, the divergent roles of PlexB motifs in distinct steps of neural development contribute to its functional versatility in neural circuit assembly.</p>
},
	keywords = {Animals, Animals, Genetically Modified, Axons, Drosophila melanogaster, Drosophila Proteins, Nerve Tissue Proteins, Neurogenesis, Olfactory Bulb, Receptors, Cell Surface, Semaphorins, Signal Transduction, Smell},
	issn = {2050-084X},
	doi = {10.7554/eLife.48594},
	author = {Guajardo, Ricardo and Luginbuhl, David J and Han, Shuo and Luo, Liqun and Li, Jiefu}
}
