@article {69559,
	title = {Nanoscale volumetric fluorescence imaging via photochemical sectioning},
	journal = {bioRxiv},
	year = {2024},
	month = {2024 Aug 05},
	abstract = {<p>Optical nanoscopy of intact biological specimens has been transformed by recent advancements in hydrogel-based tissue clearing and expansion, enabling the imaging of cellular and subcellular structures with molecular contrast. However, existing high-resolution fluorescence microscopes have limited imaging depth, which prevents the study of whole-mount specimens without physical sectioning. To address this challenge, we developed {\textquotedblleft}photochemical sectioning,{\textquotedblright} a spatially precise, light-based sample sectioning process. By combining photochemical sectioning with volumetric lattice light-sheet imaging and petabyte-scale computation, we imaged and reconstructed axons and myelination sheaths across entire mouse olfactory bulbs at nanoscale resolution. An olfactory-bulb-wide analysis of myelinated and unmyelinated axons revealed distinctive patterns of axon degeneration and de-/dysmyelination in the neurodegenerative mouse, highlighting the potential for peta- to exabyte-scale super-resolution studies using this approach.</p>
},
	doi = {10.1101/2024.08.01.605857},
	url = {https://doi.org/10.1101/2024.08.01.605857},
	author = {Wang, Wei and Ruan, Xiongtao and Liu, Gaoxiang and Milkie, Daniel E. and Li, Wenping and Betzig, Eric and Upadhyayula, Srigokul and Gao, Ruixuan}
}
