@article {69674,
	title = {Tracking fructose 1,6-bisphosphate dynamics in liver cancer cells using a fluorescent biosensor},
	journal = {iScience},
	volume = {27},
	year = {2024},
	month = {2024 Nov 20},
	pages = {111336},
	abstract = {<p>Summary HYlight is a genetically encoded fluorescent biosensor that ratiometrically monitors fructose 1,6-bisphosphate (FBP), a key glycolytic metabolite. Given the role of glucose in liver cancer metabolism, we expressed HYlight in human liver cancer cells and primary mouse hepatocytes. Through in\&nbsp;vitro, in silico, and in cellulo experiments, we showed HYlight{\textquoteright}s ability to monitor FBP changes linked to glycolysis, not gluconeogenesis. HYlight{\textquoteright}s affinity for FBP was \~{}1\&nbsp;μM and stable within physiological pH range. HYlight demonstrated weak binding to dihydroxyacetone phosphate, and its ratiometric response was influenced by both ionic strength and phosphate. Therefore, simulating cytosolic conditions in\&nbsp;vitro was necessary to establish a reliable correlation between HYlight{\textquoteright}s cellular responses and FBP concentrations. FBP concentrations were found to be in the lower micromolar range, far lower than previous millimolar estimates. Altogether, this biosensor approach offers real-time monitoring of FBP concentrations at single-cell resolution, making it an invaluable tool for the understanding of cancer metabolism.</p>
},
	keywords = {biochemistry methods, cancer, metabolomics},
	issn = {2589-0042},
	doi = {https://doi.org/10.1016/j.isci.2024.111336},
	url = {https://www.sciencedirect.com/science/article/pii/S2589004224025616},
	author = {Israel P{\'e}rez-Ch{\'a}vez and John N. Koberstein and Julia Malo Pueyo and Eduardo H. Gilglioni and Didier Vertommen and Nicolas Baeyens and Daria Ezerina and Esteban N. Gurzov and Joris Messens}
}
