@article {69749,
	title = {Cell type-specific driver lines targeting the Drosophila central complex and their use to investigate neuropeptide expression and sleep regulation},
	journal = {elife},
	year = {2025},
	month = {2025 Jan 21},
	abstract = {<p>The central complex (CX) plays a key role in many higher-order functions of the insect brain including navigation and activity regulation. Genetic tools for manipulating individual cell types, and knowledge of what neurotransmitters and neuromodulators they express, will be required to gain mechanistic understanding of how these functions are implemented. We generated and characterized split-GAL4 driver lines that express in individual or small subsets of about half of CX cell types. We surveyed neuropeptide and neuropeptide receptor expression in the central brain using fluorescent\&nbsp;<em>in situ</em>\&nbsp;hybridization. About half of the neuropeptides we examined were expressed in only a few cells, while the rest were expressed in dozens to hundreds of cells. Neuropeptide receptors were expressed more broadly and at lower levels. Using our GAL4 drivers to mark individual cell types, we found that 51 of the 85 CX cell types we examined expressed at least one neuropeptide and 21 expressed multiple neuropeptides. Surprisingly, all co-expressed a small neurotransmitter. Finally, we used our driver lines to identify CX cell types whose activation affects sleep, and identified other central brain cell types that link the circadian clock to the CX. The well-characterized genetic tools and information on neuropeptide and neurotransmitter expression we provide should enhance studies of the CX.</p>
},
	doi = {10.7554/elife.104764.2},
	url = {http://dx.doi.org/10.7554/eLife.104764.2},
	author = {Wolff, Tanya and Eddison, Mark and Chen, Nan and Nern, Aljoscha and Sundaramurthi, Preeti and Sitaraman, Divya and Rubin, Gerald M}
}
