@article {70012,
	title = {Sexually-dimorphic neurons in the Drosophila whole-brain connectome},
	journal = {Research Square},
	year = {2025},
	month = {2025 Jun 26},
	abstract = {<p>Sexual dimorphisms are present across brains. Male and female brains contain sets of cell types with differences in cell number, morphology, or synaptic connectivity between the two sexes. These differences are driven by differentially-expressed transcription factors, which set the stage for disparate sexual and social behaviors observed between males and females, such as courtship, aggression, receptivity, and mating. In the Drosophila brain, sexual dimorphisms result from differential expression of two transcription factors, Fruitless (Fru) and Doublesex (Dsx), and genetic reagents driven by enhancers for Fru and Dsx label sexually-dimorphic neurons in both male and female brains. The recent release of the first whole-brain connectome for Drosophila provides a unique opportunity to study the connectivity between these neurons as well as their integration into the larger brain network. Here, we identify 91 putative Fru or Dsx cell types, comprising ~1400 neurons, within the whole-brain connectome, using morphological similarity between electron microscopic (EM) reconstructions and light microscopic (LM) images of known Fru and Dsx neurons. We discover that while Fru and Dsx neurons are highly interconnected, each cell type typically receives more inputs from and sends more outputs to non-Fru/Dsx neurons. We characterize the connectivity in the Fru/Dsx networks to predict the function of cell types not previously characterized, we measure distances to the sensory periphery and uncover multisensory interactions, and we map connections to descending neurons that drive behavior. All Fru and Dsx labels reported here are shared within FlyWire Codex (codex.flywire.ai; gene==Fruitless or Doublesex); this work is a critical first step towards deciphering the neural basis of sexually-dimorphic behaviors and for making comparisons with future connectomes of the male brain.</p>
},
	doi = {10.21203/rs.3.rs-6881911/v1},
	url = {https://doi.org/10.21203/rs.3.rs-6881911/v1},
	author = {Deutsch, David and Matsliah, Arie and Wang, Kaiyu and Dorkenwald, Sven and Mondal, Arpita and Burke, Austin and Hebditch, James and Gager, Jay and Yu, Szi-Chieh and Sterling, Amy and McKellar, Amy and Schlegel, Philipp and Gerhard, Stephan and Sterne, Gabriella and Costa, Marta and Eichler, Katharina and Yin, Yijie and Jefferis, Gregory and Dickson, Barry and Seung, H Sebastian and Murthy, Mala}
}
