@article {70221,
	title = {A chemically switchable synthetic condensate platform for reversible protein sequestration and release},
	journal = {ACS Chem Biol},
	year = {2025},
	month = {2025 Dec 14},
	abstract = {<p>Creating artificial organelles that sequester and release specific proteins in response to a small molecule in mammalian cells is an attractive approach for regulating protein function. In this work, by combining phase-separated condensates formed by the tandem fusion of two oligomeric proteins with a trimethoprim (TMP)-responsive nanobody switch for GFP (LAMA; ligand-modulated antibody fragment), we developed a synthetic condensate system that initially sequesters GFP-tagged proteins within condensates and rapidly releases them into the cytoplasm upon TMP treatment. The released proteins can then be resequestered by washing out the TMP. This system enabled user-defined, temporal, rapid, and reversible control of cellular processes, including membrane ruffling mediated by exogenously expressed GFP-Vav2 and modulation of the cellular localization of endogenous ERK2-GFP generated by genome knock-in. Our results highlight the utility of the LAMA-based synthetic condensate platform as a novel, chemically switchable tool for regulating protein function through controlled protein sequestration and release in mammalian cells.</p>
},
	issn = {1554-8937},
	doi = {10.1021/acschembio.5c00719},
	url = {https://doi.org/10.1021/acschembio.5c00719},
	author = {Fukaya, Yoko and Yoshikawa, Masaru and Aoki, Kazuhiro and Farrants, Helen and Johnsson, Kai and Tsukiji, Shinya}
}
