@article {70494,
	title = {miR-34/449 miRNAs regulate choroid plexus ciliogenesis to control cerebrospinal fluid production},
	journal = {bioRxiv},
	year = {2026},
	month = {2026 Sep 1},
	abstract = {<p>A developmental increase in cerebrospinal fluid (CSF) production during development is essential for neuronal growth and ventricular expansion. A key regulator of CSF production is the specialized sensory multicilia of the choroid plexus (ChP), which mediate non-canonical Sonic hedgehog (Shh) signaling to suppress water channel and ion transporter expression, thereby limiting CSF production. ChP multicilia progressively shortens during development, attenuating Shh signaling and promoting CSF production. Here, we identify miR-34/449 miRNAs as essential regulators of ChP multiciliogenesis. Whereas mutations in canonical ciliogenesis genes elevate CSF production and contribute to hydrocephaly, deletion of miR-34/449 reduces CSF volume and causes microcephaly. Loss of miR-34/449 miRNAs causes excessive basal body amplification, defective basal body docking, and failure of developmental multiciliary shortening. Consequently, miR-34/449-deficient ChP cilia remain abnormally long and fail to attenuate Shh signaling, resulting in sustained repression of water channel and ion transporter expression and reduced CSF production. Mechanistically, miR-34/449 miRNAs directly target Gmnc, a master transcriptional regulator of multiciliogenesis, to restrain basal body amplification and promote basal body docking. Together, our findings identify miR-34/449 miRNAs as critical regulators of ChP multiciliogenesis and establish the developmental remodeling of ChP multicilia as a mechanism to couple Shh signaling dynamics to developmental control of CSF production.</p>
},
	doi = {10.64898/2026.08.30.747939},
	url = {https://www.biorxiv.org/content/early/2026/09/01/2026.08.30.747939},
	author = {Mao, Suifang and Song, Rui and Jovanovic, Aleksandra and Jin, Shibo and Pang, Song and Jorgens, Danielle M and Wendland, Michael F and Zimmerman, Adam and Lin, David and Xuan, Zhenyu and Xu, C Shan and Hess, Harald F and Upadhyayula, Srigokul and He, Lin}
}
