@article {65197,
	title = {Superresolution architecture of cornerstone focal adhesions in human pluripotent stem cells.},
	journal = {Nature Communications},
	volume = {10},
	year = {2019},
	month = {2019 Oct 18},
	pages = {4756},
	abstract = {<p>While it is clear that key transcriptional programmes are important for maintaining pluripotency, the requirement for cell adhesion to the extracellular matrix remains poorly defined. Human pluripotent stem cells\&nbsp;(hPSCs) form colonies encircled by an actin ring and large stable cornerstone focal adhesions (FA). Using superresolution two-colour interferometric photo-activated localisation microscopy, we examine the three-dimensional architecture of cornerstone adhesions and report vertical lamination of FA proteins with three main structural features distinct from previously studied focal adhesions: 1) integrin β5 and talin are present at high density, at the edges of cornerstone FA, adjacent to a vertical kank-rich protein wall, 2) vinculin localises higher than previously reported, displaying a head-above-tail orientation, and 3) surprisingly, actin and α-actinin are present in two discrete z-layers. Finally, we report that depletion of kanks diminishes FA patterning, and actin organisation within the colony, indicating a role for kanks in hPSC colony architecture.</p>
},
	issn = {2041-1723},
	doi = {10.1038/s41467-019-12611-w},
	author = {Stubb, Aki and Guzm{\'a}n, Camilo and N{\"a}rv{\"a}, Elisa and Aaron, Jesse and Chew, Teng-Leong and Saari, Markku and Miihkinen, Mitro and Jacquemet, Guillaume and Ivaska, Johanna}
}
