@article {65241,
	title = {Dual-plane 3-photon microscopy with remote focusing.},
	journal = {Biomedical Optics Express},
	volume = {10},
	year = {2019},
	month = {2019 Nov 1},
	pages = {5585-5599},
	abstract = {<p>3-photon excitation enables fluorescence microscopy deep in densely labeled and highly scattering samples. To date, 3-photon excitation has been restricted to scanning a single focus, limiting the speed of volume acquisition. Here, for the first time to our knowledge, we implemented and characterized dual-plane 3-photon microscopy with temporal multiplexing and remote focusing, and performed simultaneous calcium imaging of two planes beyond 600 {\textmu}m deep in the cortex of a pan-excitatory GCaMP6s transgenic mouse with a per-plane framerate of 7 Hz and an effective 2 MHz laser repetition rate. This method is a straightforward and generalizable modification to single-focus 3PE systems, doubling the rate of volume (column) imaging with off-the-shelf components and minimal technical constraints.</p>
},
	issn = {2156-7085},
	doi = {10.1364/BOE.10.005585},
	author = {Takasaki, Kevin T and Tsyboulski, Dmitri and Waters, Jack}
}
