@article {69517,
	title = {Resolution in super-resolution microscopy - definition, trade-offs and perspectives.},
	journal = {Nat Rev Mol Cell Biol},
	year = {2024},
	month = {2024 Jul 01},
	abstract = {<p>Super-resolution microscopy (SRM) is gaining popularity in biosciences; however, claims about optical resolution are contested and often misleading. In this Viewpoint, experts share their views on resolution and common trade-offs, such as labelling and post-processing, aiming to clarify them for biologists and facilitate deeper understanding and best use of SRM.</p>
},
	issn = {1471-0080},
	doi = {10.1038/s41580-024-00755-7},
	author = {Prakash, Kirti and Baddeley, David and Eggeling, Christian and Fiolka, Reto and Heintzmann, Rainer and Manley, Suliana and Radenovic, Aleksandra and Smith, Carlas and Shroff, Hari and Schermelleh, Lothar}
}
@article {65262,
	title = {Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging.},
	journal = {Nature Methods},
	volume = {17},
	year = {2020},
	month = {2020 Jan 06},
	pages = {225-231},
	abstract = {<p>Combining the molecular specificity of fluorescent probes with three-dimensional imaging at nanoscale resolution is critical for investigating the spatial organization and interactions of cellular organelles and protein complexes. We present a 4Pi single-molecule switching super-resolution microscope that enables ratiometric multicolor imaging of mammalian cells at 5-10-nm localization precision in three dimensions using {\textquoteright}salvaged fluorescence{\textquoteright}. Imaging two or three fluorophores simultaneously, we show fluorescence images that resolve the highly convoluted Golgi apparatus and the close contacts between the endoplasmic reticulum and the plasma membrane, structures that have traditionally been the imaging realm of electron microscopy. The salvaged fluorescence approach is equally applicable in most single-objective microscopes.</p>
},
	issn = {1548-7105},
	doi = {10.1038/s41592-019-0676-4},
	author = {Zhang, Yongdeng and Schroeder, Lena K and Lessard, Mark D and Kidd, Phylicia and Chung, Jeeyun and Song, Yuanbin and Benedetti, Lorena and Li, Yiming and Ries, Jonas and Grimm, Jonathan B and Lavis, Luke D and De Camilli, Pietro and Rothman, James E and Baddeley, David and Bewersdorf, Joerg}
}
