@article {65972,
	title = {High throughput instrument to screen fluorescent proteins under two-photon excitation.},
	journal = {Biomedical Optics Express},
	volume = {11},
	year = {2020},
	month = {2020 Dec 01},
	pages = {7192-7203},
	abstract = {<p>Two-photon microscopy together with fluorescent proteins and fluorescent protein-based biosensors are commonly used tools in neuroscience. To enhance their experimental scope, it is important to optimize fluorescent proteins for two-photon excitation. Directed evolution of fluorescent proteins under one-photon excitation is common, but many one-photon properties do not correlate with two-photon properties. A simple system for expressing fluorescent protein mutants is colonies on an agar plate. The small focal volume of two-photon excitation makes creating a high throughput screen in this system a challenge for a conventional point-scanning approach. We present an instrument and accompanying software that solves this challenge by selectively scanning each colony based on a colony map captured under one-photon excitation. This instrument, called the GIZMO, can measure the two-photon excited fluorescence of 10,000 colonies in 7 hours. We show that the GIZMO can be used to evolve a fluorescent protein under two-photon excitation.</p>
},
	issn = {2156-7085},
	doi = {10.1364/BOE.409353},
	author = {Molina, Rosana S and King, Jonathan and Franklin, Jacob and Clack, Nathan and McRaven, Christopher and Goncharov, Vasily and Flickinger, Daniel and Svoboda, Karel and Drobizhev, Mikhail and Hughes, Thomas E}
}
@article {65377,
	title = {High-throughput cellular-resolution synaptic connectivity mapping in vivo with concurrent two-photon optogenetics and volumetric Ca2+ imaging},
	journal = {bioRxiv},
	year = {2020},
	month = {2020 Feb 25},
	abstract = {<p>The ability to measure synaptic connectivity and properties is essential for understanding neuronal circuits. However, existing methods that allow such measurements at cellular resolution are laborious and technically demanding. Here, we describe a system that allows such measurements in a high-throughput way by combining two-photon optogenetics and volumetric Ca2+\&nbsp;imaging with whole-cell recording. We reveal a circuit motif for generating fast undulatory locomotion in zebrafish.</p>
},
	doi = {https://doi.org/10.1101/2020.02.21.959650},
	author = {McRaven, Christopher and Tanese, Dimitrii and Zhang, Lixia and Yang, Chao-Tsung and Ahrens, Misha B and Emiliani, Valentina and Koyama, Minoru}
}
