@article {70189,
	title = {Relative phase of membrane potential theta oscillations between individual hippocampal neurons code space},
	journal = {bioRxiv},
	year = {2025},
	month = {2025 Nov 14},
	abstract = {<p>The timing of spikes dictates a neuron{\textquoteright}s impact on downstream circuits and behavior, and spike timing is determined by the membrane potential (Vm). However, due to technical challenges, it has been impossible to analyze the relative timing of Vm dynamics between neurons during behavior. Using large scale membrane voltage imaging, we simultaneously recorded Vm from many individual hippocampal neurons in animals engaged in a virtual spatial task. We found that relative phase of Vm theta oscillations across neurons exhibit gradual or discrete shifts depending on spatial position. This finding extends beyond previous studies showing Vm dynamics in single neurons or spiking activity in multiple neurons, revealing previously unknown evidence for consistent coding of space by spike-independent relative phase of Vm theta dynamics between neurons.</p>
},
	doi = {10.1101/2025.11.14.688496},
	url = {https://www.biorxiv.org/content/early/2025/11/14/2025.11.14.688496},
	author = {Athif, Mohamed and Malmberg, Samantha and Mount, Rebecca and Patel, Kush and Wang, Yangyang and Shaw, Dana and Ravasio, Cara and Xiao, Sheng and Rillosi, Enrico and Tseng, Hua-An and Plutkis, Sarah E. and Grimm, Jonathan B. and Lavis, Luke D. and Mertz, Jerome and Han, Xue and Hasselmo, Michael E.}
}
@article {65634,
	title = {Precision Calcium Imaging of Dense Neural Populations via a Cell-Body-Targeted Calcium Indicator.},
	journal = {Neuron},
	volume = {107},
	year = {2020},
	month = {2020 Aug 01},
	pages = {470},
	abstract = {<p>Methods for one-photon fluorescent imaging of calcium dynamics can capture the activity of hundreds of neurons across large fields of view at a low equipment complexity and cost. In contrast to two-photon methods, however, one-photon methods suffer from higher levels of crosstalk from neuropil, resulting in a decreased signal-to-noise ratio and artifactual correlations of neural activity. We address this problem by engineering cell-body-targeted variants of the fluorescent calcium indicators GCaMP6f and GCaMP7f. We screened fusions of GCaMP to natural, as well as artificial, peptides and identified fusions that localized GCaMP to within 50\&nbsp;μm of the cell body of neurons in mice and larval zebrafish. One-photon imaging of soma-targeted GCaMP in dense neural circuits reported fewer artifactual spikes from neuropil, an increased signal-to-noise ratio, and decreased artifactual correlation across neurons. Thus, soma-targeting of fluorescent calcium indicators facilitates usage of simple, powerful, one-photon methods for imaging neural calcium dynamics.</p>
},
	issn = {1097-4199},
	doi = {10.1016/j.neuron.2020.05.029},
	author = {Shemesh, Or A and Linghu, Changyang and Piatkevich, Kiryl D and Goodwin, Daniel and Celiker, Orhan Tunc and Gritton, Howard J and Romano, Michael F and Gao, Ruixuan and Yu, Chih-Chieh Jay and Tseng, Hua-An and Bensussen, Seth and Narayan, Sujatha and Yang, Chao-Tsung and Freifeld, Limor and Siciliano, Cody A and Gupta, Ishan and Wang, Joyce and Pak, Nikita and Yoon, Young-Gyu and Ullmann, Jeremy F P and Guner-Ataman, Burcu and Noamany, Habiba and Sheinkopf, Zoe R and Park, Won Min and Asano, Shoh and Keating, Amy E and Trimmer, James S and Reimer, Jacob and Tolias, Andreas S and Bear, Mark F and Tye, Kay M and Han, Xue and Ahrens, Misha B and Boyden, Edward S}
}
