@article {69424, title = {Adaptive optical third-harmonic generation microscopy for in vivo imaging of tissues}, journal = {bioRxiv}, year = {2024}, month = {2024 May 05}, abstract = {

Third-harmonic generation microscopy is a powerful label-free nonlinear imaging technique, providing essential information about structural characteristics of cells and tissues without requiring external labelling agents. In this work, we integrated a recently developed compact adaptive optics module into a third-harmonic generation microscope, to measure and correct for optical aberrations in complex tissues. Taking advantage of the high sensitivity of the third-harmonic generation process to material interfaces and thin membranes, along with the 1,300-nm excitation wavelength used here, our adaptive optical third-harmonic generation microscope enabled high-resolution in vivo imaging within highly scattering biological model systems. Examples include imaging of myelinated axons and vascular structures within the mouse spinal cord and deep cortical layers of the mouse brain, along with imaging of key anatomical features in the roots of the model plant Brachypodium distachyon. In all instances, aberration correction led to significant enhancements in image quality.

}, doi = {10.1101/2024.05.02.592275}, url = {https://www.biorxiv.org/content/early/2024/05/05/2024.05.02.592275}, author = {Cristina Rodr{\'\i}guez and Daisong Pan and Ryan G. Natan and Manuel A. Mohr and Max Miao and Xiaoke Chen and Trent R. Northen and John P. Vogel and Na Ji} } @article {65895, title = {An adaptive optics module for deep tissue multiphoton imaging in vivo}, journal = {Nature Methods}, year = {2021}, month = {2021 Oct 04}, pages = {1259-64}, abstract = {

Understanding complex biological systems requires visualizing structures and processes deep within living organisms. We developed a compact adaptive optics module and incorporated it into two- and three-photon fluorescence microscopes, to measure and correct tissue-induced aberrations. We resolved synaptic structures in deep cortical and subcortical areas of the mouse brain, and demonstrated high-resolution imaging of neuronal structures and somatosensory-evoked calcium responses in the mouse spinal cord at unprecedented depths\ in vivo.

}, doi = {10.1038/s41592-021-01279-0}, author = {Cristina Rodr{\'\i}guez and Anderson Chen and Jos{\'e} A. Rivera and Manuel A. Mohr and Yajie liang and Wenzhi Sun and Daniel E. Milkie and Thomas G. Bifano and Xiaoke Chen and Na Ji} }