@article {69044, title = {Blue-shifted genetically encoded Ca2+ indicator with enhanced two-photon absorption}, journal = {Neurophotonics}, year = {2024}, month = {2024 Apr 11}, abstract = {

Significance:\ Genetically encoded calcium ion (Ca2+) indicators (GECIs) are powerful tools for monitoring intracellular\ Ca2+\ concentration changes in living cells and model organisms. In particular, GECIs have found particular utility for monitoring the transient increase of\ Ca2+concentration that is associated with the neuronal action potential. However, the palette of highly optimized GECIs for imaging of neuronal activity remains relatively limited. Expanding the selection of available GECIs to include new colors and distinct photophysical properties could create new opportunities for\ in vitro\ and\ in vivo\ fluorescence imaging of neuronal activity. In particular, blue-shifted variants of GECIs are expected to have enhanced two-photon brightness, which would facilitate multiphoton microscopy.

Aim:\ We describe the development and applications of T-GECO1-a high-performance blue-shifted GECI based on the\ Clavularia sp.-derived mTFP1.

Approach:\ We use protein engineering and extensive directed evolution to develop T-GECO1. We characterize the purified protein and assess its performance\ in vitro\ using one-photon excitation in cultured rat hippocampal neurons,\ in vivo\ using one-photon excitation fiber photometry in mice, and\ ex vivo\ using two-photon\ Ca2+\ imaging in hippocampal slices.

Results:\ The\ Ca2+-bound state of T-GECO1 has an excitation peak maximum of 468 nm, an emission peak maximum of 500 nm, an extinction coefficient of\ 49,300M-1cm-1, a quantum yield of 0.83, and two-photon brightness approximately double that of EGFP. The\ Ca2+-dependent fluorescence increase is 15-fold, and the apparent\ Kd\ for\ Ca2+\ is 82 nM. With two-photon excitation conditions at 850 nm, T-GECO1 consistently enabled the detection of action potentials with higher signal-to-noise (SNR) than a late generation GCaMP variant.

Conclusions:\ T-GECO1 is a high-performance blue-shifted GECI that, under two-photon excitation conditions, provides advantages relative to late generation GCaMP variants.

Keywords:\ blue-shifted fluorescence; genetically encoded calcium ion indicator; neuronal activity imaging; protein engineering; two-photon excitation.

}, doi = {10.1117/1.NPh.11.2.024207}, url = {https://doi.org/10.1117/1.NPh.11.2.024207}, author = {Abhi Aggarwal and Smrithi Sunil and Imane Bendifallah and Michael Moon and Mikhail Drobizhev and Landon Zarowny and Jihong Zheng and Sheng-Yi Wu and Alexander W. Lohman and Alison G. Tebo and Valentina Emiliani and Kaspar Podgorski and Yi Shen and Robert E. Campbell} }