@article {70450,
	title = {Self-supervised Internal Learning Enhances Isotropic Resolution for Three-dimensional Fluorescence Microscopy},
	journal = {bioRxiv},
	year = {2026},
	month = {2026 Jun 08},
	abstract = {<p>Three dimensional fluorescence microscopy often exhibits anisotropic resolution because axial information is poorly sampled and more blurred than lateral information, which complicates quantitative interpretation of fine 3D structures. Although optical remedies and computational restoration have been explored, many approaches require demanding system calibration or rely on accurate PSF models and assumptions that are difficult to satisfy across all samples and modalities. Here we present DeepIso, a self-supervised isotropy restoration framework that couples supervised pretraining with an internal-learning inference stage to estimate degradation directly from the measured volume. Without explicit PSF specification or enforced lateral-axial structural equivalence, DeepIso recovers axial frequency content and improves the continuity of elongated structures while retaining fine features, with superior performance over existing computational approaches in terms of both visual inspection and quantitative metrics. The method is validated on synthetic benchmarks and experimental datasets, demonstrating isotropy enhancement across confocal, light-sheet, and 3D structured illumination microscopy, thereby supporting downstream volumetric analysis including segmentation and tracking.</p>
},
	doi = {10.64898/2026.06.04.717237},
	url = {https://www.biorxiv.org/content/early/2026/06/08/2026.06.04.717237},
	author = {Wei, Mingzhe and Xu, Pengcheng and Liu, Junyu and Li, Xuesong and Feng, Xuhui and Zhu, Jun and Dong, Renwei and Ran, Hengjia and Zhu, Wentao and Han, Yubing and Li, Yue and Guo, Min and Liu, Huafeng}
}
@article {69297,
	title = {Cell division machinery drives cell-specific gene activation during differentiation in .},
	journal = {Proc Natl Acad Sci U S A},
	volume = {121},
	year = {2024},
	month = {2024 Mar 6},
	pages = {e2400584121},
	abstract = {<p>When faced with starvation, the bacterium transforms itself into a dormant cell type called a "spore". Sporulation initiates with an asymmetric division event, which requires the relocation of the core divisome components FtsA and FtsZ, after which the sigma factor σ is exclusively activated in the smaller daughter cell. Compartment-specific activation of σ requires the SpoIIE phosphatase, which displays a biased localization on one side of the asymmetric division septum and associates with the structural protein DivIVA, but the mechanism by which this preferential localization is achieved is unclear. Here, we isolated a variant of DivIVA that indiscriminately activates σ in both daughter cells due to promiscuous localization of SpoIIE, which was corrected by overproduction of FtsA and FtsZ. We propose that the core components of the redeployed cell division machinery drive the asymmetric localization of DivIVA and SpoIIE to trigger the initiation of the sporulation program.</p>
},
	issn = {1091-6490},
	doi = {10.1073/pnas.2400584121},
	author = {Chareyre, Sylvia and Li, Xuesong and Anjuwon-Foster, Brandon R and Updegrove, Taylor B and Clifford, Sarah and Brogan, Anna P and Su, Yijun and Zhang, Lixia and Chen, Jiji and Shroff, Hari and Ramamurthi, Kumaran S}
}
