@article {69093, title = {Decoding life{\textquoteright}s inner workings: advances in quantitative bioimaging.}, journal = {Open Biology}, volume = {13}, year = {2023}, month = {2023 Nov 29}, pages = {230329}, abstract = {

This special feature of , titled {\textquoteright}Advances in Quantitative Bioimaging{\textquoteright}, proposes an overview of the latest advancements in quantitative bioimaging techniques and their wide-ranging applications. The articles cover various topics, including modern imaging methods that enable visualization on a nanoscale, such as super-resolution microscopy and single-particle analysis. These techniques offer unparalleled insights into complex molecular structures and dynamic cellular processes , such as mapping nuclear pore proteins or tracking single histone deposition events throughout the cell cycle. The articles presented in this edition showcase cutting-edge quantitative imaging techniques coupled with advanced computational analysis capable of precisely measuring biological structures and processes. Examples range from correlating calcium release events to underlying protein organization in heart cells to pioneering tools for categorizing changes in microglia morphology under various conditions. This editorial highlights how these advancements are revolutionizing our understanding of living systems, while acknowledging challenges that must be addressed to fully exploit the potential of these emerging technologies, such as improving molecular probes, algorithms and correlation protocols.

}, keywords = {Algorithms, Microscopy, Fluorescence, Molecular Structure}, issn = {2046-2441}, doi = {10.1098/rsob.230329}, author = {Henriques, Ricardo and Leterrier, Christophe and Weigel, Aubrey} } @article {69076, title = {High-fidelity 3D live-cell nanoscopy through data-driven enhanced super-resolution radial fluctuation.}, journal = {Nature Methods}, year = {2023}, month = {2023 Nov 13}, abstract = {

Live-cell super-resolution microscopy enables the imaging of biological structure dynamics below the diffraction limit. Here we present enhanced super-resolution radial fluctuations (eSRRF), substantially improving image fidelity and resolution compared to the original SRRF method. eSRRF incorporates automated parameter optimization based on the data itself, giving insight into the trade-off between resolution and fidelity. We demonstrate eSRRF across a range of imaging modalities and biological systems. Notably, we extend eSRRF to three dimensions by combining it with multifocus microscopy. This realizes live-cell volumetric super-resolution imaging with an acquisition speed of ~1 volume per second. eSRRF provides an accessible super-resolution approach, maximizing information extraction across varied experimental conditions while minimizing artifacts. Its optimal parameter prediction strategy is generalizable, moving toward unbiased and optimized analyses in super-resolution microscopy.

}, issn = {1548-7105}, doi = {10.1038/s41592-023-02057-w}, author = {Laine, Romain F and Heil, Hannah S and Coelho, Simao and Nixon-Abell, Jonathon and Jimenez, Ang{\'e}lique and Wiesner, Theresa and Mart{\'\i}nez, Dami{\'a}n and Galgani, Tommaso and R{\'e}gnier, Louise and Stubb, Aki and Follain, Gautier and Webster, Samantha and Goyette, Jesse and Dauphin, Aurelien and Salles, Audrey and Culley, Si{\^a}n and Jacquemet, Guillaume and Hajj, Bassam and Leterrier, Christophe and Henriques, Ricardo} } @article {6489, title = {PALM and STORM: unlocking live-cell super-resolution.}, journal = {Biopolymers}, volume = {95}, year = {2011}, month = {2011 May}, pages = {322-31}, abstract = {

Live-cell fluorescence light microscopy has emerged as an important tool in the study of cellular biology. The development of fluorescent markers in parallel with super-resolution imaging systems has pushed light microscopy into the realm of molecular visualization at the nanometer scale. Resolutions previously only attained with electron microscopes are now within the grasp of light microscopes. However, until recently, live-cell imaging approaches have eluded super-resolution microscopy, hampering it from reaching its full potential for revealing the dynamic interactions in biology occurring at the single molecule level. Here we examine recent advances in the super-resolution imaging of living cells by reviewing recent breakthroughs in single molecule localization microscopy methods such as PALM and STORM to achieve this important goal.

}, keywords = {Buffers, Fluorescence, Fluorescent Dyes, Luminescent Proteins, Microscopy, Nanotechnology, Photochemical Processes, Single-Cell Analysis}, issn = {0006-3525}, doi = {10.1002/bip.21586}, author = {Henriques, Ricardo and Griffiths, Caron and Hesper Rego, E and Mhlanga, Musa M} }