@article {70359,
	title = {Adapting upright light sheet fluorescence microscopy for imaging at air-liquid interface},
	journal = {bioRxiv},
	year = {2026},
	month = {2026 Apr 09},
	abstract = {<p>Light sheet fluorescence microscopy (LSFM) is increasingly appreciated as the gold standard for gentle, volumetric imaging with fast acquisition speeds and/or long imaging durations. However, the often-constrained sample space of these microscopes has precluded a specific class of biological specimens from being studied with these tools: those requiring an air-liquid interface (ALI). Here, we present a device for robust imaging at ALI on an upright light sheet microscope with dipping objectives. We demonstrate the system using three relevant use-cases: ex vivo embryonic mouse salivary glands, human epidermal equivalent cultures, and in vivo adult Drosophila melanogaster brains. While the device presented is engineered for one specific light sheet microscope design, it provides a blueprint for easy adaptation to other systems. In doing so, it can potentially spur the use of LSFM for model systems that have so far been unable to take advantage of this powerful technology.</p>
},
	doi = {10.64898/2026.04.07.716945},
	url = {https://www.biorxiv.org/content/early/2026/04/09/2026.04.07.716945},
	author = {Hobson, Chad M. and Izumi, Kenji and Aaron, Jesse S. and Bharathan, Navaneetha Krishnan and Ceriani, M. Fernanda and Giang, William and Ispizua, Juan I. and Kowalczyk, Andrew P. and Lee, Rachel M. and Morales, E. Angelo and Puls, Owen F. and Quarles, Ellen and Rodriguez-Caron, Micaela and Stahley, Sara N. and Tassara, Francisco and Wang, Shaohe and Yao, Shunze and Tsuchiya, Takanori and Chew, Teng-Leong}
}
@book {69492,
	title = {Salivary gland developmental mechanics},
	series = {Current Topics in Developmental Biology},
	year = {2024},
	publisher = {Elsevier},
	organization = {Elsevier},
	abstract = {<p>The salivary gland undergoes branching morphogenesis to elaborate into a tree-like structure with numerous saliva-secreting acinar units, all joined by a hierarchical ductal system. The expansive epithelial surface generated by branching morphogenesis serves as the structural basis for the efficient production and delivery of saliva. Here, we elucidate the process of salivary gland morphogenesis, emphasizing the role of mechanics. Structurally, the developing salivary gland is characterized by a stratified epithelium tightly encased by the basement membrane, which is in turn surrounded by a mesenchyme consisting of a dense network of interstitial matrix and mesenchymal cells. Diverse cell types and extracellular matrices bestow this developing organ with organized, yet spatially varied mechanical properties. For instance, the surface epithelial sheet of the bud is highly fluidic due to its high cell motility and weak cell-cell adhesion, rendering it highly pliable. In contrast, the inner core of the bud is more rigid, characterized by reduced cell motility and strong cell-cell adhesion, which likely provide structural support for the tissue. The interactions between the surface epithelial sheet and the inner core give rise to budding morphogenesis. Furthermore, the basement membrane and the mesenchyme offer mechanical constraints that could play a pivotal role in determining the higher-order architecture of a fully mature salivary gland.</p>
},
	issn = {00702153},
	doi = {10.1016/bs.ctdb.2024.05.002},
	url = {https://doi.org/10.1016/bs.ctdb.2024.05.002},
	author = {Morales, E. Angelo and Wang, Shaohe}
}
