@article {70405,
	title = {Resolving protein condensates and aggregates in vivo by boxcar-enhanced Fluorescence-detected mid-Infrared photothermaL Microscopy (FILM)},
	journal = {Commun Chem},
	year = {2026},
	month = {2026 May 11},
	abstract = {<p>Protein assemblies, including aggregates and condensates, are closely linked to health and diseases. We demonstrate boxcar-enhanced Fluorescence-detected mid-Infrared photothermaL Microscopy (FILM), using two model species, Caenorhabditis elegans and Saccharomyces cerevisiae, to quantitatively resolve these protein states in vivo by imaging β-sheet and α-helix secondary structures and analyzing their ratios. This method directly distinguishes polyglutamine (PolyQ) protein aggregates, α-synuclein protein condensates, and P-granule condensates implicated in neurodegenerative diseases and embryonic development in live organisms. It further enables the unraveling of protein assembly dynamics and their physio-pathological roles, such as age-related progression of PolyQ from condensates to aggregates.</p>
},
	issn = {2399-3669},
	doi = {10.1038/s42004-026-01970-3},
	url = {https://doi.org/10.1038/s42004-026-01970-3},
	author = {Weinberg, Bethany and Guo, Zhongyue and Tang, Rong and Ao, Jianpeng and Yin, Jiaze and Ding, Guangrui and Blute, Todd A and Savini, Marzia and Chiesa, Giulio and Wang, Wentao and Good, Matt and Wang, Meng C and Cheng, Ji-Xin}
}
