@article {4275,
	title = {A bright and photostable photoconvertible fluorescent protein.},
	journal = {Nature Methods},
	volume = {6},
	year = {2009},
	month = {2009 Feb},
	pages = {131-3},
	abstract = {<p>Photoconvertible fluorescent proteins are potential tools for investigating dynamic processes in living cells and for emerging super-resolution microscopy techniques. Unfortunately, most probes in this class are hampered by oligomerization, small photon budgets or poor photostability. Here we report an EosFP variant that functions well in a broad range of protein fusions for dynamic investigations, exhibits high photostability and preserves the approximately 10-nm localization precision of its parent.</p>
},
	keywords = {Animals, Fibroblasts, Fluorescence, Fluorescent Dyes, Hela Cells, Humans, Luminescent Proteins, Microscopy, Mutagenesis, Photochemical Processes, Recombinant Fusion Proteins, Site-Directed},
	issn = {1548-7105},
	doi = {10.1038/nmeth.1296},
	author = {McKinney, Sean A and Murphy, Christopher S and Hazelwood, Kristin L and Davidson, Michael W and Looger, Loren L}
}
@article {1646,
	title = {Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators.},
	journal = {Nature Methods},
	volume = {6},
	year = {2009},
	month = {2009 Dec},
	pages = {875-81},
	abstract = {<p>Genetically encoded calcium indicators (GECIs) can be used to image activity in defined neuronal populations. However, current GECIs produce inferior signals compared to synthetic indicators and recording electrodes, precluding detection of low firing rates. We developed a single-wavelength GCaMP2-based GECI (GCaMP3), with increased baseline fluorescence (3-fold), increased dynamic range (3-fold) and higher affinity for calcium (1.3-fold). We detected GCaMP3 fluorescence changes triggered by single action potentials in pyramidal cell dendrites, with signal-to-noise ratio and photostability substantially better than those of GCaMP2, D3cpVenus and TN-XXL. In Caenorhabditis elegans chemosensory neurons and the Drosophila melanogaster antennal lobe, sensory stimulation-evoked fluorescence responses were significantly enhanced with GCaMP3 (4-6-fold). In somatosensory and motor cortical neurons in the intact mouse, GCaMP3 detected calcium transients with amplitudes linearly dependent on action potential number. Long-term imaging in the motor cortex of behaving mice revealed large fluorescence changes in imaged neurons over months.</p>
},
	keywords = {Animals, Brain, Caenorhabditis elegans, Calcium, Cell Line, Drosophila melanogaster, Fluorescence Resonance Energy Transfer, Humans, Mice, Neurons},
	issn = {1548-7105},
	doi = {10.1038/nmeth.1398},
	author = {Tian, Lin and Hires, S Andrew and Mao, Tianyi and Huber, Daniel and Chiappe, M Eugenia and Chalasani, Sreekanth H and Petreanu, Leopoldo and Akerboom, Jasper and McKinney, Sean A and Schreiter, Eric R and Bargmann, Cornelia I and Jayaraman, Vivek and Svoboda, Karel and Looger, Loren L}
}
