@article {5232,
	title = {Elucidation of the regulation of an adult cuticle gene Acp65A by the transcription factor Broad.},
	journal = {Insect Molecular Biology},
	volume = {18},
	year = {2009},
	month = {2009 Aug},
	pages = {421-9},
	abstract = {<p>Broad (BR), an ecdysone-inducible transcription factor, is a major determinant of the pupal stage. The misexpression of BR-Z1 isoform (BR-Z1) during adult development of Drosophila melanogaster prevents the expression of the adult cuticle protein 65A gene (Acp65A). We found that the proximal 237 bp of the 5{\textquoteright} flanking region of Acp65A were sufficient to mediate this suppression. A targeted point mutation of a putative BR-Z1 response element (BRE) within this region showed that it was not involved. Drosophila hormone receptor-like 38 (DHR38) is required for Acp65A expression. We found that BR-Z1 repressed DHR38 expression and that BR{\textquoteright}s inhibition of Acp65A expression was rescued by exogenous expression of DHR38. Thus, BR-Z1 suppresses Acp65A expression by preventing the normal up-regulation of DHR38 at the time of adult cuticle formation.</p>
},
	keywords = {Animals, Base Sequence, Binding Sites, Developmental, Drosophila melanogaster, Drosophila Proteins, Gene Expression Regulation, Genetic, Hot Temperature, Insect Proteins, Integumentary System, Molecular Sequence Data, Promoter Regions, Protein Binding, Pupa, Transcription Factors},
	issn = {1365-2583},
	doi = {10.1111/j.1365-2583.2009.00889.x},
	author = {Cui, H-Y and Lestradet, M and Bruey-Sedano, N and Charles, J-P and Riddiford, L M}
}
