@article {2504,
	title = {Kr{\"u}ppel homolog 1 (Kr-h1) mediates juvenile hormone action during metamorphosis of Drosophila melanogaster.},
	journal = {Mechanisms of Development},
	volume = {125},
	year = {2008},
	month = {2008 Jan-Feb},
	pages = {91-105},
	abstract = {<p>Juvenile hormone (JH) given at pupariation inhibits bristle formation and causes pupal cuticle formation in the abdomen of Drosophila melanogaster due to its prolongation of expression of the transcription factor Broad (BR). In a microarray analysis of JH-induced gene expression in abdominal integument, we found that Kr{\"u}ppel homolog 1 (Kr-h1) was up-regulated during most of adult development. Quantitative real-time PCR analyses showed that Kr-h1 up-regulation began at 10h after puparium formation (APF), and Kr-h1 up-regulation occurred in imaginal epidermal cells, persisting larval muscles, and larval oenocytes. Ectopic expression of Kr-h1 in abdominal epidermis using T155-Gal4 to drive UAS-Kr-h1 resulted in missing or short bristles in the dorsal midline. This phenotype was similar to that seen after a low dose of JH or after misexpression of br between 21 and 30 h APF. Ectopic expression of Kr-h1 prolonged the expression of BR protein in the pleura and the dorsal tergite. No Kr-h1 was seen after misexpression of br. Thus, Kr-h1 mediates some of the JH signaling in the adult abdominal epidermis and is upstream of br in this pathway. We also show for the first time that the JH-mediated maintenance of br expression in this epidermis is patterned and that JH delays the fusion of the imaginal cells and the disappearance of Dpp in the dorsal midline.</p>
},
	keywords = {Animals, Base Sequence, Biological, DNA Primers, Drosophila melanogaster, Drosophila Proteins, Kruppel-Like Transcription Factors, Larva, Metamorphosis, Oligonucleotide Array Sequence Analysis, Polymerase Chain Reaction, Up-Regulation},
	issn = {0925-4773},
	doi = {10.1016/j.mod.2007.10.002},
	author = {Minakuchi, Chieka and Zhou, Xiaofeng and Riddiford, Lynn M}
}
