@article {2400,
	title = {Transcription of histone gene cluster by differential core-promoter factors.},
	journal = {Genes \& Development},
	volume = {21},
	year = {2007},
	month = {2007 Nov 15},
	pages = {2936-49},
	abstract = {<p>The 100 copies of tandemly arrayed Drosophila linker (H1) and core (H2A/B and H3/H4) histone gene cluster are coordinately regulated during the cell cycle. However, the molecular mechanisms that must allow differential transcription of linker versus core histones prevalent during development remain elusive. Here, we used fluorescence imaging, biochemistry, and genetics to show that TBP (TATA-box-binding protein)-related factor 2 (TRF2) selectively regulates the TATA-less Histone H1 gene promoter, while TBP/TFIID targets core histone transcription. Importantly, TRF2-depleted polytene chromosomes display severe chromosomal structural defects. This selective usage of TRF2 and TBP provides a novel mechanism to differentially direct transcription within the histone cluster. Moreover, genome-wide chromatin immunoprecipitation (ChIP)-on-chip analyses coupled with RNA interference (RNAi)-mediated functional studies revealed that TRF2 targets several classes of TATA-less promoters of \&gt;1000 genes including those driving transcription of essential chromatin organization and protein synthesis genes. Our studies establish that TRF2 promoter recognition complexes play a significantly more central role in governing metazoan transcription than previously appreciated.</p>
},
	keywords = {Animals, Base Sequence, Biological, Cells, Chromatin Immunoprecipitation, Chromosomes, Cluster Analysis, Cultured, Drosophila, Firefly, Fluorescence, Genes, Genetic, Histones, Immunohistochemistry, In Situ Hybridization, Insect, Luciferases, Models, Molecular Sequence Data, Multigene Family, Nucleic Acid, Promoter Regions, Reporter, RNA Interference, Sequence Homology, TATA Box Binding Protein-Like Proteins, TATA-Box Binding Protein, Transcription},
	issn = {0890-9369},
	doi = {10.1073/pnas.1100640108},
	author = {Isogai, Yoh and Keles, S{\"u}nd{\"u}z and Prestel, Matthias and Hochheimer, Andreas and Tjian, Robert}
}
@article {2424,
	title = {Diversified transcription initiation complexes expand promoter selectivity and tissue-specific gene expression.},
	journal = {Genes \& Development},
	volume = {17},
	year = {2003},
	month = {2003 Jun 1},
	pages = {1309-20},
	keywords = {Animals, Developmental, Drosophila, Drosophila Proteins, Gene Expression Regulation, Genetic, Male, Messenger, Organ Specificity, Promoter Regions, RNA, RNA Polymerase III, Spermatogenesis, TATA Box Binding Protein-Like Proteins, Telomeric Repeat Binding Protein 2, Transcription},
	issn = {0890-9369},
	doi = {10.1073/pnas.1100640108},
	author = {Hochheimer, Andreas and Tjian, Robert}
}
@article {2427,
	title = {TRF2 associates with DREF and directs promoter-selective gene expression in Drosophila.},
	journal = {Nature},
	volume = {420},
	year = {2002},
	month = {2002 Nov 28},
	pages = {439-45},
	abstract = {<p>Drosophila TATA-box-binding protein (TBP)-related factor 2 (TRF2) is a member of a family of TBP-related factors present in metazoan organisms. Recent evidence suggests that TRF2s are required for proper embryonic development and differentiation. However, true target promoters and the mechanisms by which TRF2 operates to control transcription remain elusive. Here we report the antibody affinity purification of a Drosophila TRF2-containing complex that contains components of the nucleosome remodelling factor (NURF) chromatin remodelling complex as well as the DNA replication-related element (DRE)-binding factor DREF. This latter finding led us to potential target genes containing TRF2-responsive promoters. We have used a combination of in vitro and in vivo assays to show that the DREF-containing TRF2 complex directs core promoter recognition of the proliferating cell nuclear antigen (PCNA) gene. We also identified additional TRF2-responsive target genes involved in DNA replication and cell proliferation. These data suggest that TRF2 functions as a core promoter-selectivity factor responsible for coordinating transcription of a subset of genes in Drosophila.</p>
},
	keywords = {Animals, Cell Line, Chromatin, Drosophila, Drosophila Proteins, Gene Expression Regulation, Genes, Genetic, Insect, Macromolecular Substances, Nuclease Protection Assays, Oligonucleotide Array Sequence Analysis, Precipitin Tests, Proliferating Cell Nuclear Antigen, Promoter Regions, Protein Binding, RNA Interference, Substrate Specificity, Telomeric Repeat Binding Protein 2, Transcription Factors, Transcription Initiation Site},
	issn = {0028-0836},
	doi = {10.1073/pnas.1100640108},
	author = {Hochheimer, Andreas and Zhou, Sharleen and Zheng, Shuang and Holmes, Michael C and Tjian, Robert}
}
