@article {958,
	title = {Comparative genomics of the eukaryotes.},
	journal = {Science},
	volume = {287},
	year = {2000},
	month = {2000 Mar 24},
	pages = {2204-15},
	abstract = {<p>A comparative analysis of the genomes of Drosophila melanogaster, Caenorhabditis elegans, and Saccharomyces cerevisiae-and the proteins they are predicted to encode-was undertaken in the context of cellular, developmental, and evolutionary processes. The nonredundant protein sets of flies and worms are similar in size and are only twice that of yeast, but different gene families are expanded in each genome, and the multidomain proteins and signaling pathways of the fly and worm are far more complex than those of yeast. The fly has orthologs to 177 of the 289 human disease genes examined and provides the foundation for rapid analysis of some of the basic processes involved in human disease.</p>
},
	keywords = {Animals, Apoptosis, Biological Evolution, Caenorhabditis elegans, Cell Adhesion, Cell Cycle, Drosophila melanogaster, Duplicate, Fungal Proteins, Genes, Genetic Diseases, Genetics, Genome, Helminth Proteins, Humans, Immunity, Inborn, Insect Proteins, Medical, Multigene Family, Neoplasms, Protein Structure, Proteome, Saccharomyces cerevisiae, Signal Transduction, Tertiary},
	issn = {0036-8075},
	doi = {10.1186/gb-2007-8-7-r145},
	author = {Rubin, G M and Yandell, M D and Wortman, J R and Gabor Miklos, G L and Nelson, C R and Hariharan, I K and Fortini, M E and Li, P W and Apweiler, R and Fleischmann, W and Cherry, J M and Henikoff, S and Skupski, M P and Misra, S and Ashburner, M and Birney, E and Boguski, M S and Brody, T and Brokstein, P and Celniker, S E and Chervitz, S A and Coates, D and Cravchik, A and Gabrielian, A and Galle, R F and Gelbart, W M and George, R A and Goldstein, L S and Gong, F and Guan, P and Harris, N L and Hay, B A and Hoskins, R A and Li, J and Li, Z and Hynes, R O and Jones, S J and Kuehl, P M and Lemaitre, B and Littleton, J T and Morrison, D K and Mungall, C and O{\textquoteright}Farrell, P H and Pickeral, O K and Shue, C and Vosshall, L B and Zhang, J and Zhao, Q and Zheng, X H and Lewis, S}
}
@article {959,
	title = {The Berkeley Drosophila genome project gene disruption project: single P-element insertions mutating 25\% of vital Drosophila genes.},
	journal = {Genetics},
	volume = {153},
	year = {1999},
	month = {1999 Sep},
	pages = {135-77},
	abstract = {<p>A fundamental goal of genetics and functional genomics is to identify and mutate every gene in model organisms such as Drosophila melanogaster. The Berkeley Drosophila Genome Project (BDGP) gene disruption project generates single P-element insertion strains that each mutate unique genomic open reading frames. Such strains strongly facilitate further genetic and molecular studies of the disrupted loci, but it has remained unclear if P elements can be used to mutate all Drosophila genes. We now report that the primary collection has grown to contain 1045 strains that disrupt more than 25\% of the estimated 3600 Drosophila genes that are essential for adult viability. Of these P insertions, 67\% have been verified by genetic tests to cause the associated recessive mutant phenotypes, and the validity of most of the remaining lines is predicted on statistical grounds. Sequences flanking \&gt;920 insertions have been determined to exactly position them in the genome and to identify 376 potentially affected transcripts from collections of EST sequences. Strains in the BDGP collection are available from the Bloomington Stock Center and have already assisted the research community in characterizing \&gt;250 Drosophila genes. The likely identity of 131 additional genes in the collection is reported here. Our results show that Drosophila genes have a wide range of sensitivity to inactivation by P elements, and provide a rationale for greatly expanding the BDGP primary collection based entirely on insertion site sequencing. We predict that this approach can bring \&gt;85\% of all Drosophila open reading frames under experimental control.</p>
},
	keywords = {Alleles, Animals, California, Crosses, DNA, DNA Transposable Elements, Drosophila melanogaster, Essential, Expressed Sequence Tags, Female, Genes, Genetic, Genetic Linkage, Genome, Insect, Insertional, Male, Models, Mutagenesis, Mutation, Phenotype, Recessive, Reproducibility of Results, Sequence Analysis},
	issn = {0016-6731},
	doi = {10.1186/gb-2007-8-7-r145},
	author = {Spradling, A C and Stern, D and Beaton, A and Rhem, E J and Laverty, T and Mozden, N and Misra, S and Rubin, G M}
}
