@article {1192, title = {Regulation of nuclear genes encoding mitochondrial proteins in Saccharomyces cerevisiae.}, journal = {Journal of Bacteriology}, volume = {177}, year = {1995}, month = {1995 Dec}, pages = {6836-43}, abstract = {

Selection for mutants which release glucose repression of the CYB2 gene was used to identify genes which regulate repression of mitochondrial biogenesis. We have identified two of these as the previously described GRR1/CAT80 and ROX3 genes. Mutations in these genes not only release glucose repression of CYB2 but also generally release respiration of the mutants from glucose repression. In addition, both mutants are partially defective in CYB2 expression when grown on nonfermentable carbon sources, indicating a positive regulatory role as well. ROX3 was cloned by complementation of a glucose-inducible flocculating phenotype of an amber mutant and has been mapped as a new leftmost marker on chromosome 2. The ROX3 mutant has only a modest defect in glucose repression of GAL1 but is substantially compromised in galactose induction of GAL1 expression. This mutant also has increased SUC2 expression on nonrepressing carbon sources. We have also characterized the regulation of CYB2 in strains carrying null mutation in two other glucose repression genes, HXK2 and SSN6, and show that HXK2 is a negative regulator of CYB2, whereas SSN6 appears to be a positive effector of CYB2 expression.

}, keywords = {Base Sequence, Cell Compartmentation, Cell Nucleus, Chromosome Mapping, Chromosomes, Fermentation, Fungal, Fungal Proteins, Gene Expression Regulation, Genes, Genetic, Glucose, Mitochondria, Molecular Sequence Data, Mutagenesis, Oxygen Consumption, Saccharomyces cerevisiae, Selection, Telomere}, issn = {0021-9193}, author = {Brown, T A and Evangelista, C and Trumpower, B L} } @article {1193, title = {Strain-dependent variation in carbon source regulation of nucleus-encoded mitochondrial proteins of Saccharomyces cerevisiae.}, journal = {Journal of Bacteriology}, volume = {177}, year = {1995}, month = {1995 Mar}, pages = {1380-2}, abstract = {

Nuclear genes encoding mitochondrial proteins are regulated by carbon source with significant heterogeneity among four Saccharomyces cerevisiae strains. This strain-dependent variation is seen both in respiratory capacity of the cells and in the expression of beta-galactosidase reporter fusions to the promoters of CYB2, CYC1, CYC3, MnSOD, and RPO41.

}, keywords = {Bacterial Proteins, Base Sequence, beta-Galactosidase, Carbon, Cell Nucleus, Cytochrome b Group, Cytochrome c Group, Ferritins, Fungal, Fungal Proteins, Gene Expression Regulation, Genes, Genetic, Genetic Variation, L-Lactate Dehydrogenase, L-Lactate Dehydrogenase (Cytochrome), Mitochondria, Molecular Sequence Data, Oxygen Consumption, Promoter Regions, Recombinant Fusion Proteins, Reporter, Saccharomyces cerevisiae, Species Specificity, Superoxide Dismutase}, issn = {0021-9193}, author = {Brown, T A and Trumpower, B L} } @article {1195, title = {Isolation and characterization of QCR9, a nuclear gene encoding the 7.3-kDa subunit 9 of the Saccharomyces cerevisiae ubiquinol-cytochrome c oxidoreductase complex. An intron-containing gene with a conserved sequence occurring in the intron of COX4.}, journal = {The Journal of Biological Chemistry}, volume = {265}, year = {1990}, month = {1990 Dec 5}, pages = {20813-21}, abstract = {

A nuclear gene (QCR9) encoding the 7.3-kDa subunit 9 of the mitochondrial cytochrome bc1 complex from Saccharomyces cerevisiae has been isolated from a yeast genomic library by hybridization with a degenerate oligonucleotide corresponding to nine amino acids proximal to the N terminus of purified subunit 9. QCR9 includes a 195-base pair open reading frame capable of encoding a protein of 66 amino acids and having a predicted molecular weight of 7471. The N-terminal methionine of subunit 9 is removed posttranslationally because the N-terminal sequence of the purified protein begins with serine 2. The ATG triplet corresponding to the N-terminal methionine is separated from the open reading frame by an intron. The intron is 213 base pairs long and contains previously reported 5{\textquoteright} donor, 3{\textquoteright} acceptor, and TACTAAC sequences necessary for splicing. The splice junctions, as well as the 5{\textquoteright} end of the message, were confirmed by isolation and sequencing of a cDNA copy of QCR9. In addition, the intron contains a nucleotide sequence in which 15 out of 18 nucleotides are identical with a sequence in the intron of COX4, the nuclear gene encoding cytochrome c oxidase subunit 4. The deduced amino acid sequence of the yeast subunit 9 is 39\% identical with that of a protein of similar molecular weight from beef heart cytochrome bc1 complex. If conservative substitutions are allowed for, the two proteins are 56\% similar. The predicted secondary structure of the 7.3-kDa protein revealed a single possible transmembrane helix, in which the amino acids conserved between beef heart and yeast are asymmetrically arranged along one face of the helix, implying that this domain of the protein is involved in a conserved interaction with another hydrophobic protein of the cytochrome bc1 complex. Two yeast strains, JDP1 and JDP2, were constructed in which QCR9 was deleted. Both strains grew very poorly, or not at all, on nonfermentable carbon sources and exhibited, at most, only 5\% of wild-type ubiquinol-cytochrome c oxidoreductase activity. Optical spectra of mitochondrial membranes from the deletion strains revealed slightly reduced levels of cytochrome b. When JDP1 and JDP2 were complemented with a plasmid carrying QCR9, the resulting yeast grew normally on ethanol/glycerol and exhibited normal cytochrome c reductase activities and optical spectra. These results indicate that QCR9 encodes a 7.3-kDa subunit of the bc1 complex that is required for formation of a fully functional complex.(ABSTRACT TRUNCATED AT 400 WORDS)

}, keywords = {Amino Acid Sequence, Base Sequence, Cell Nucleus, Chromosome Deletion, Cloning, Electron Transport Complex III, Electron Transport Complex IV, Fungal, Genes, Genomic Library, Introns, Macromolecular Substances, Models, Molecular, Molecular Sequence Data, Protein Conformation, Restriction Mapping, Saccharomyces cerevisiae}, issn = {0021-9258}, author = {Phillips, J D and Schmitt, M E and Brown, T A and Beckmann, J D and Trumpower, B L} } @article {1194, title = {A rapid and simple method for preparation of RNA from Saccharomyces cerevisiae.}, journal = {Nucleic Acids Research}, volume = {18}, year = {1990}, month = {1990 May 25}, pages = {3091-2}, keywords = {Agar Gel, Electrophoresis, Fungal, Methods, RNA, Saccharomyces cerevisiae}, issn = {0305-1048}, author = {Schmitt, M E and Brown, T A and Trumpower, B L} }