@article {1179,
	title = {DNA primase of human mitochondria is associated with structural RNA that is essential for enzymatic activity.},
	journal = {Cell},
	volume = {45},
	year = {1986},
	month = {1986 Jun 20},
	pages = {817-25},
	abstract = {<p>DNA primase isolated from human mitochondria sediments in glycerol density gradients at 30S and 70S. These unusually high sedimentation coefficients are a result of association of the primase activity with RNA. Treatment of primase with nuclease not only affects its sedimentation behavior, but also inactivates the primase activity. The major RNA species that cofractionates with primase activity is shown by direct sequence analysis to be cytosolic 5.8S ribosomal RNA (rRNA). Specific degradation of endogenous 5.8S rRNA using ribonuclease H and oligonucleotides complementary to 5.8S rRNA results in reduction of primase activity. Other small RNAs may play a structural role in the formation of an active DNA primase complex.</p>
},
	keywords = {Centrifugation, Density Gradient, DNA, DNA Primase, Endoribonucleases, Humans, Micrococcal Nuclease, Mitochondria, Mitochondrial, Ribonuclease H, Ribosomal, RNA, RNA Nucleotidyltransferases},
	issn = {0092-8674},
	doi = {10.1101/gad.1352105},
	author = {Wong, T W and Clayton, D A}
}
@article {1180,
	title = {In vitro replication of human mitochondrial DNA: accurate initiation at the origin of light-strand synthesis.},
	journal = {Cell},
	volume = {42},
	year = {1985},
	month = {1985 Oct},
	pages = {951-8},
	abstract = {<p>Synthesis of human light-strand mitochondrial DNA was accomplished in vitro using DNA primase, DNA polymerase, and other accessory proteins isolated from human mitochondria. Replication begins with the synthesis of primer RNA on a T-rich sequence in the origin stem-loop structure of the template DNA and absolutely requires ATP. A transition from RNA synthesis to DNA synthesis occurs near the base of the stem-loop structure and a potential recognition site for signaling that transition has been identified. The start sites of the in vitro products were mapped at the nucleotide level and were found to be in excellent agreement with those of in vivo nascent light-strand DNA. Isolated human mitochondrial enzymes recognize and utilize the bovine, but not the mouse, origin of light-strand replication.</p>
},
	keywords = {Animals, Base Sequence, Cattle, Cloning, DNA, DNA Replication, DNA Restriction Enzymes, Genetic, Humans, KB Cells, Macromolecular Substances, Mitochondrial, Molecular, Nucleic Acid Conformation, Templates},
	issn = {0092-8674},
	doi = {10.1101/gad.1352105},
	author = {Wong, T W and Clayton, D A}
}
