@article {47315, title = {Sequential ionic and conformational signaling by calcium channels drives neuronal gene expression.}, journal = {Science (New York, N.Y.)}, volume = {351}, year = {2016}, month = {2016 Feb 19}, pages = {863-7}, abstract = {

Voltage-gated CaV1.2 channels (L-type calcium channel α1C subunits) are critical mediators of transcription-dependent neural plasticity. Whether these channels signal via the influx of calcium ion (Ca2+), voltage-dependent conformational change (VΔC), or a combination of the two has thus far been equivocal. We fused CaV1.2 to a ligand-gated Ca2+-permeable channel, enabling independent control of localized Ca2+\ and VΔC signals. This revealed an unexpected dual requirement: Ca2+\ must first mobilize actin-bound Ca2+/calmodulin-dependent protein kinase II, freeing it for subsequent VΔC-mediated accumulation. Neither signal alone sufficed to activate transcription. Signal order was crucial: Efficiency peaked when Ca2+\ preceded VΔC by 10 to 20 seconds. CaV1.2 VΔC synergistically augmented signaling by\ N-methyl-D-aspartate receptors. Furthermore, VΔC mistuning correlated with autistic symptoms in Timothy syndrome. Thus, nonionic VΔC signaling is vital to the function of CaV1.2 in synaptic and neuropsychiatric processes.

}, doi = {10.1126/science.aad3647}, author = {Li, Boxing and Tadross, Michael R and Tsien, Richard W} } @article {47017, title = {Optogenetics: 10 years after ChR2 in neurons-views from the community.}, journal = {Nature Neuroscience}, volume = {18}, year = {2015}, month = {2015 Aug 26}, pages = {1202-12}, issn = {1546-1726}, doi = {10.1038/nn.4106}, author = {Adamantidis, Antoine and Arber, Silvia and Bains, Jaideep S and Bamberg, Ernst and Bonci, Antonello and Buzs{\'a}ki, Gy{\"o}rgy and Cardin, Jessica A and Costa, Rui M and Dan, Yang and Goda, Yukiko and Graybiel, Ann M and H{\"a}usser, Michael and Hegemann, Peter and Huguenard, John R and Insel, Thomas R and Janak, Patricia H and Johnston, Daniel and Josselyn, Sheena A and Koch, Christof and Kreitzer, Anatol C and L{\"u}scher, Christian and Malenka, Robert C and Miesenb{\"o}ck, Gero and Nagel, Georg and Roska, Botond and Schnitzer, Mark J and Shenoy, Krishna V and Soltesz, Ivan and Sternson, Scott M and Tsien, Richard W and Tsien, Roger Y and Turrigiano, Gina G and Tye, Kay M and Wilson, Rachel I} } @article {45064, title = {Virtual finger boosts three-dimensional imaging and microsurgery as well as terabyte volume image visualization and analysis.}, journal = {Nature Communications}, volume = {5}, year = {2014}, month = {2014 Jul 11}, pages = {4342}, abstract = {

Three-dimensional (3D) bioimaging, visualization and data analysis are in strong need of powerful 3D exploration techniques. We develop virtual finger (VF) to generate 3D curves, points and regions-of-interest in the 3D space of a volumetric image with a single finger operation, such as a computer mouse stroke, or click or zoom from the 2D-projection plane of an image as visualized with a computer. VF provides efficient methods for acquisition, visualization and analysis of 3D images for roundworm, fruitfly, dragonfly, mouse, rat and human. Specifically, VF enables instant 3D optical zoom-in imaging, 3D free-form optical microsurgery, and 3D visualization and annotation of terabytes of whole-brain image volumes. VF also leads to orders of magnitude better efficiency of automated 3D reconstruction of neurons and similar biostructures over our previous systems. We use VF to generate from images of 1,107 Drosophila GAL4 lines a projectome of a Drosophila brain.

}, issn = {2041-1723}, doi = {10.1038/ncomms5342}, author = {Peng, Hanchuan and Tang, Jianyong and Xiao, Hang and Bria, Alessandro and Zhou, Jianlong and Butler, Victoria and Zhou, Zhi and Gonzalez-Bellido, Paloma T and Oh, Seung W and Chen, Jichao and Mitra, Ananya and Tsien, Richard W and Zeng, Hongkui and Ascoli, Giorgio A and Iannello, Giulio and Hawrylycz, Michael and Myers, Eugene and Long, Fuhui} }